9F07
TUBULIN:STATHMIN:DARPIN:TAU MTBR3 COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.298, 222.250, 227.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 159.030 - 2.211 |
| R-factor | 0.2014 |
| Rwork | 0.200 |
| R-free | 0.23350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.930 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 159.032 | 2.511 |
| High resolution limit [Å] | 2.211 | 2.211 |
| Rpim | 0.082 | 0.798 |
| Number of reflections | 81231 | 4062 |
| <I/σ(I)> | 9.4 | 1.8 |
| Completeness [%] | 60.6 | |
| Redundancy | 27.4 | |
| CC(1/2) | 0.996 | 0.607 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 20 to 25% (w/v) polyethylene glycol 3350 0.2 M K/Na tartrate |
