9EWC

DNA Polymerase Lambda I493R 528-530 NEY, TTP:At Ca2+ Ground State Ternary Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	DNA polymerase lambda	polymer	330	36857.1	2	UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791)	Homo sapiens (human)	Pol Lambda,DNA polymerase beta-2,Pol beta2,DNA polymerase kappa
2	C, D (E, F)	DNA template strand	polymer	11	3374.2	2		Homo sapiens
3	E, G (G, I)	DNA primer strand upstream	polymer	6	1793.2	2		Homo sapiens
4	F, H (H, J)	DNA primer strand downstream (5'P)	polymer	4	1191.8	2		Homo sapiens
5	I, J, M (A, C)	SODIUM ION	non-polymer		23.0	3	Chemie (NA)
6	K (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
7	L (C)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
8	N, O, P (A, C, F)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A, C: 242 - 462 (UniProt: Q9UGP5)

PDB	External Database	Details
Gly 241	-	expression tag

A, C: 472 - 575 (UniProt: Q9UGP5)

PDB	External Database	Details
Lys 463	-	linker
Gly 464	-	linker
Glu 470	-	linker
Thr 471	-	linker
Arg 492	Ile 492	engineered mutation
Asn 528	Ser 528	engineered mutation
Tyr 530	His 530	engineered mutation
Ala 543	Cys 543	conflict

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	86432.7
Non-Polymers*	Number of molecules	5
	Total formula weight	171.1
All*	Total formula weight	86603.8

*Water molecules are not included.