9EWC
DNA Polymerase Lambda I493R 528-530 NEY, TTP:At Ca2+ Ground State Ternary Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-10-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 149.904, 149.904, 272.154 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.260 - 3.670 |
| R-factor | 0.2456 |
| Rwork | 0.240 |
| R-free | 0.29260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.826 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.173 | 4.018 |
| High resolution limit [Å] | 3.543 | 3.543 |
| Rpim | 0.658 | 1.192 |
| Number of reflections | 106687 | 4580 |
| <I/σ(I)> | 7.8 | 1.8 |
| Completeness [%] | 91.7 | 74.2 |
| Redundancy | 17.7 | 15.2 |
| CC(1/2) | 0.850 | 0.747 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277.15 | 20 mM bicine pH 7.5 300 mM Na-K tartrate 20% PEG 1000 |
