9EUJ

Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the post-contraction state - sheath initiator, wedge module, inner tripod, arm segment, and proximal tail sheath proteins

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (H)	Baseplate protein	polymer	194	21588.1	1	UniProt (A0A0U1WGQ5)	Staphylococcus phage 812	arm segment protein
2	B, C, D, E, F... (I, J, K, L, M...)	Major tail sheath protein	polymer	587	64559.0	6	UniProt (A0A0U1WZ79)	Staphylococcus phage 812	tail sheath protein
3	H (A)	Baseplate wedge subunit	polymer	234	26611.8	1	UniProt (A0A0U1UXD7) Pfam (PF10934)	Staphylococcus phage 812	sheath initiator protein
4	I, J (B, C)	Baseplate component	polymer	348	39248.9	2	UniProt (A0A0U1WF63) Pfam (PF04865)	Staphylococcus phage 812	wedge protein
5	K (D)	TmpF	polymer	1019	116389.9	1	UniProt (A0A0U1WGD3)	Staphylococcus phage 812	arm scaffold protein
6	L, M, N (E, F, G)	DUF4815 domain-containing protein	polymer	1152	129263.0	3	UniProt (A0A8E5NSA0)	Staphylococcus phage 812	tripod protein

Sequence modifications

H: 1 - 173 (UniProt: A0A0U1WGQ5)

PDB	External Database	Details
Asp 174	-	expression tag
Pro 175	-	expression tag
Asn 176	-	expression tag
Ser 177	-	expression tag
Ser 178	-	expression tag
Ser 179	-	expression tag
Val 180	-	expression tag
Asp 181	-	expression tag
Lys 182	-	expression tag
Leu 183	-	expression tag
Ala 184	-	expression tag
Ala 185	-	expression tag
Ala 186	-	expression tag
Leu 187	-	expression tag
Glu 188	-	expression tag
His 189	-	expression tag
His 190	-	expression tag
His 191	-	expression tag
His 192	-	expression tag
His 193	-	expression tag
His 194	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	1018230.4
All*	Total formula weight	1018230.4

*Water molecules are not included.