9EH1
RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-nucleosome, 20 bp upstream
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase subunit | polymer | 1544 | 173530.1 | 1 | UniProt (A0A8D1DS11) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) UniProt (by SIFTS) (A0A8D1DPV6) | Sus scrofa (pig) | |
| 2 | B (B) | DNA-directed RNA polymerase subunit beta | polymer | 1159 | 132189.7 | 1 | UniProt (A0A4X1TVZ5) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) UniProt (by SIFTS) (A0A0B8RVL1) | Sus scrofa (pig) | |
| 3 | C (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 269 | 30767.2 | 1 | UniProt (A0A481DF93) Pfam (PF01193) Pfam (PF01000) UniProt (by SIFTS) (I3LCH3) | Sus scrofa (pig) | |
| 4 | D (D) | RNA polymerase Rpb4/RPC9 core domain-containing protein | polymer | 126 | 14706.5 | 1 | UniProt (A0A8D0KES4) | Sus scrofa (pig) | |
| 5 | E (E) | DNA-directed RNA polymerase II subunit E | polymer | 209 | 24513.1 | 1 | UniProt (A0A4X1VTX4) Pfam (PF03871) Pfam (PF01191) UniProt (by SIFTS) (I3LSI7) | Sus scrofa (pig) | RPB5 homolog |
| 6 | F (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 78 | 8978.6 | 1 | UniProt (A0A8D1QTB4) Pfam (PF01192) UniProt (by SIFTS) (A0A4X1VEK9) | Sus scrofa (pig) | DNA-directed RNA polymerase II subunit F,RPB6 homolog |
| 7 | G (G) | DNA-directed RNA polymerase subunit | polymer | 171 | 19227.2 | 1 | UniProt (A0A4X1VKG7) Pfam (PF03876) Pfam (PF00575) | Sus scrofa (pig) | |
| 8 | H (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 149 | 17031.1 | 1 | UniProt (I3LCB2) Pfam (PF03870) | Sus scrofa (pig) | |
| 9 | I (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 116 | 13521.2 | 1 | UniProt (P60899) Pfam (PF02150) Pfam (PF01096) | Sus scrofa (pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 10 | J (J) | DNA-directed RNA polymerase I, II, and III subunit RPABC5 | polymer | 66 | 7525.9 | 1 | Pfam (PF01194) UniProt (by SIFTS) (A0A8W4F9W9) | Sus scrofa | |
| 11 | K (K) | DNA-directed RNA polymerase II subunit RPB11-a | polymer | 115 | 13068.0 | 1 | UniProt (F1RKE4) Pfam (PF13656) | Sus scrofa (pig) | |
| 12 | L (L) | RNA polymerase II subunit K | polymer | 47 | 5747.8 | 1 | UniProt (A0A8D0JYF1) Pfam (PF03604) UniProt (by SIFTS) (A0A4X1TRS6) | Sus scrofa (pig) | |
| 13 | M (M) | Transcription elongation factor SPT6 | polymer | 1002 | 117027.7 | 1 | Homo sapiens | ||
| 14 | N (N) | Non-template DNA | polymer | 170 | 52348.3 | 1 | synthetic construct | ||
| 15 | O (O) | Protein IWS1 homolog | polymer | 132 | 14972.5 | 1 | UniProt (Q96ST2) Pfam (PF08711) | Homo sapiens (human) | IWS1-like protein |
| 16 | P (P) | RNA | polymer | 11 | 3475.1 | 1 | synthetic construct | ||
| 17 | Q (Q) | RNA polymerase-associated protein CTR9 homolog | polymer | 890 | 102859.1 | 1 | UniProt (Q6PD62) Pfam (PF13374) Pfam (PF13181) | Homo sapiens (human) | SH2 domain-binding protein 1 |
| 18 | R (R) | RNA polymerase-associated protein RTF1 homolog | polymer | 248 | 29128.2 | 1 | UniProt (Q92541) Pfam (PF03126) | Homo sapiens (human) | |
| 19 | S (S) | Transcription elongation factor A protein 1 | polymer | 170 | 19371.1 | 1 | UniProt (P23193) Pfam (PF07500) Pfam (PF01096) UniProt (by SIFTS) (B7Z4W0) | Homo sapiens (human) | Transcription elongation factor S-II protein 1,Transcription elongation factor TFIIS.o |
| 20 | T (T) | Template DNA | polymer | 181 | 56053.8 | 1 | synthetic construct | ||
| 21 | U (U) | RNA polymerase-associated protein LEO1 | polymer | 125 | 14451.3 | 1 | Homo sapiens | ||
| 22 | V (V) | RNA polymerase II-associated factor 1 homolog | polymer | 244 | 28991.5 | 1 | Homo sapiens | ||
| 23 | W (W) | Superkiller complex protein 8, N-terminally processed | polymer | 300 | 33029.8 | 1 | UniProt (Q9GZS3) Pfam (PF00400) | Homo sapiens (human) | WD repeat-containing protein 61,N-terminally processed |
| 24 | X (X) | Parafibromin | polymer | 43 | 5029.8 | 1 | UniProt (Q6P1J9) UniProt (by SIFTS) (A0A2R8YHB3) | Homo sapiens (human) | Cell division cycle protein 73 homolog,Hyperparathyroidism 2 protein |
| 25 | Y (Y) | Transcription elongation factor SPT4 | polymer | 116 | 13079.0 | 1 | UniProt (P63272) Pfam (PF06093) | Homo sapiens (human) | hSPT4,DRB sensitivity-inducing factor 14 kDa subunit,DSIF p14,DRB sensitivity-inducing factor small subunit,DSIF small subunit |
| 26 | Z (Z) | Transcription elongation factor SPT5 | polymer | 510 | 57815.3 | 1 | Homo sapiens | ||
| 27 | AA, EA (a, e) | Histone H3 | polymer | 136 | 15437.1 | 2 | UniProt (A0A310TTQ1) Pfam (PF00125) | Xenopus laevis (African clawed frog) | |
| 28 | BA, FA (b, f) | Histone H4 | polymer | 78 | 8853.3 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 29 | CA, GA (c, g) | Histone H2A type 1 | polymer | 130 | 14109.4 | 2 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) | Xenopus laevis (African clawed frog) | |
| 30 | DA, HA (d, h) | Histone H2B 1.1 | polymer | 123 | 13655.9 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 31 | IA (l) | Histone-lysine N-methyltransferase SETD2 | polymer | 589 | 67378.5 | 1 | UniProt (Q9BYW2) Pfam (PF17907) Pfam (PF00856) | Homo sapiens (human) | HIF-1,Huntingtin yeast partner B,Huntingtin-interacting protein 1,HIP-1,Huntingtin-interacting protein B,Lysine N-methyltransferase 3A,Protein-lysine N-methyltransferase SETD2,SET domain-containing protein 2,hSET2,p231HBP |
| 32 | JA, KA, MA, NA, OA... (A, B, C, I, J...) | ZINC ION | non-polymer | 65.4 | 9 | Chemie (ZN) | |||
| 33 | LA (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
a, e: 0 - 135 (UniProt: A0A310TTQ1)
c, g: 0 - 129 (UniProt: P06897)
d, h: 4 - 125 (UniProt: P02281)
| PDB | External Database | Details |
|---|---|---|
| Met 36 | Lys 37 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 99 | Gly 100 | conflict |
| Ser 123 | Ala 124 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 3 | - | initiating methionine |
| Thr 32 | Ser 33 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 35 |
| Total formula weight | 1179929.1 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 613.0 | |
| All* | Total formula weight | 1180542.1 |
