9EGV

HOIL-1 RING2 domain bound to ubiquitin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	RanBP-type and C3HC4-type zinc finger-containing protein 1	polymer	88	9594.1	2	UniProt (Q9BYM8)	Homo sapiens (human)	HBV-associated factor 4,Heme-oxidized IRP2 ubiquitin ligase 1,HOIL-1,Hepatitis B virus X-associated protein 4,RING finger protein 54,RING-type E3 ubiquitin transferase HOIL-1,Ubiquitin-conjugating enzyme 7-interacting protein 3
2	C (C)	Polyubiquitin-C	polymer	88	9894.3	1	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (human)
3	D, E, F, H, I... (A, B)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
4	G (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5	K, L, M (B, C)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
6	N, O, P (A, B, C)	water	water		18.0	103	Chemie (HOH)

Sequence modifications

A, B: 425 - 510 (UniProt: Q9BYM8)

PDB	External Database	Details
Gly 423	-	expression tag
Pro 424	-	expression tag
Ala 460	Cys 460	engineered mutation

C: 0 - 76 (UniProt: P0CG48)

PDB	External Database	Details
Met -11	-	initiating methionine
Ala -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Ala -2	-	expression tag
Ala -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	29082.5
Non-Polymers*	Number of molecules	10
	Total formula weight	716.1
All*	Total formula weight	29798.6

*Water molecules are not included.