9EGV

HOIL-1 RING2 domain bound to ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	I 41 2 2
Unit cell lengths	95.922, 95.922, 187.429
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.960 - 2.000
R-factor	0.18
Rwork	0.178
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.063
Data reduction software	XDS (Nov 1, 2016 BUILT=20161205)
Data scaling software	Aimless (0.7.8)
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.960	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.225	2.644
Rmeas	0.234	2.760
Rpim	0.064	0.783
Total number of observations	398102	25259
Number of reflections	29960	2098
<I/σ(I)>	8.6	0.8
Completeness [%]	99.7
Redundancy	13.3	12
CC(1/2)	0.998	0.421

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	0.01 M magnesium chloride hexahydrate, 0.05 M MES monohydrate pH 6.2, 1.8 M lithium sulfate monohydrate