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9ED9

SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State III - checkpoint)

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(B)
sgRNApolymer9731335.61synthetic construct
2B
(C)
PAM-distal Target Strandpolymer3310077.51synthetic construct
3C
(D)
Non-target strandpolymer5517027.01synthetic construct
4D
(c)
PAM-proximal Target Strandpolymer226743.41synthetic construct
5E
(A)
CRISPR-associated endonuclease Cas9/Csn1polymer1368158699.81UniProt (Q99ZW2)Streptococcus pyogenesSpCas9,SpyCas9
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains5
Total formula weight223883.3
All*Total formula weight223883.3
*Water molecules are not included.

237735

PDB entries from 2025-06-18

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