9ED9
SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State III - checkpoint)
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOCONTINUUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.95 |
| Software | PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
