9EBX
Chimeric fluorescence biosensor formed from a lactate-binding protein and GFP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Green fluorescent protein,Methyl-accepting chemotaxis transducer (TlpC) | polymer | 517 | 58441.0 | 1 | UniProt (P42212) UniProt (O24911) | Aequorea victoria | |
| 2 | B (A) | water | water | 18.0 | 9 | Chemie (HOH) |
Sequence modifications
A: 11 - 153 (UniProt: P42212)
A: 159 - 421 (UniProt: O24911)
A: 426 - 515 (UniProt: P42212)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Lys 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| His 9 | - | expression tag |
| Val 10 | - | expression tag |
| Leu 73 | Phe 64 | conflict |
| Swg 74 | Ser 65 | chromophore |
| Swg 74 | Tyr 66 | chromophore |
| Swg 74 | Gly 67 | chromophore |
| Ala 79 | Ser 72 | conflict |
| Ser 152 | Tyr 145 | conflict |
| Ser 153 | Asn 146 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Asp 159 | Ser 30 | conflict |
| Pro 160 | Tyr 31 | conflict |
| Phe 161 | Lys 32 | conflict |
| Thr 162 | Val 33 | conflict |
| Ser 422 | - | linker |
| Ser 423 | - | linker |
| His 424 | - | linker |
| Ser 425 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Gly 154 | - | linker |
| Arg 155 | - | linker |
| Thr 156 | - | linker |
| Gly 157 | - | linker |
| Ile 158 | - | linker |
| Thr 430 | Met 153 | conflict |
| Ala 440 | Val 163 | conflict |
| Gly 452 | Ser 175 | conflict |
| Lys 483 | Ala 206 | conflict |
| Leu 508 | His 231 | conflict |
| Gln 515 | Lys 238 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 58441.0 | |
| All* | Total formula weight | 58441.0 |
