9EBX

Chimeric fluorescence biosensor formed from a lactate-binding protein and GFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978532
Spacegroup name	P 1
Unit cell lengths	34.350, 57.980, 67.319
Unit cell angles	97.82, 91.43, 104.24

Refinement procedure

Resolution	46.130 - 2.420
R-factor	0.2386
Rwork	0.234
R-free	0.27960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.242
Data reduction software	XDS (0.7.15)
Data scaling software	Aimless (1.19)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487: 000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.130	2.507
High resolution limit [Å]	2.420	2.420
Rmerge	0.031	0.371
Rmeas	0.044	0.525
Rpim	0.031	0.371
Number of reflections	16566	1775
<I/σ(I)>	12.56	1.75
Completeness [%]	86.9	92.11
Redundancy	1.9	1.9
CC(1/2)	0.999	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	magnesium chloride hexahydrate, HEPES, PEG 3350