9EBW

Chimeric fluorescence biosensor formed from a lactate-binding protein and GFP, bound to lactate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Green fluorescent protein,Methyl-accepting chemotaxis transducer (TlpC)	polymer	517	58441.0	4	UniProt (P42212) UniProt (O24911)	Aequorea victoria
2	E, G (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	F, H (B, C)	LACTIC ACID	non-polymer		90.1	2	Chemie (LAC)
4	I, J, K, L (A, B, C, D)	water	water		18.0	119	Chemie (HOH)

Sequence modifications

A, B, C, D: 11 - 153 (UniProt: P42212)

PDB	External Database	Details
Met 1	-	initiating methionine
Lys 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Val 10	-	expression tag
Leu 73	Phe 64	conflict
Swg 74	Ser 65	chromophore
Swg 74	Tyr 66	chromophore
Swg 74	Gly 67	chromophore
Ala 79	Ser 72	conflict
Ser 152	Tyr 145	conflict
Ser 153	Asn 146	conflict

A, B, C, D: 159 - 421 (UniProt: O24911)

PDB	External Database	Details
Asp 159	Ser 30	conflict
Pro 160	Tyr 31	conflict
Phe 161	Lys 32	conflict
Thr 162	Val 33	conflict
Ser 422	-	linker
Ser 423	-	linker
His 424	-	linker
Ser 425	-	linker

A, B, C, D: 426 - 515 (UniProt: P42212)

PDB	External Database	Details
Gly 154	-	linker
Arg 155	-	linker
Thr 156	-	linker
Gly 157	-	linker
Ile 158	-	linker
Thr 430	Met 153	conflict
Ala 440	Val 163	conflict
Gly 452	Ser 175	conflict
Lys 483	Ala 206	conflict
Leu 508	His 231	conflict
Gln 515	Lys 238	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	233763.9
Non-Polymers*	Number of molecules	4
	Total formula weight	364.3
All*	Total formula weight	234128.2

*Water molecules are not included.