9E9S
Cryo-EM structure of the PGD2-bound prostaglandin D2 receptor (DP1)-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37799.2 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (D) | Prostaglandin D2 receptor | polymer | 385 | 43222.9 | 1 | UniProt (Q13258) Pfam (PF00001) | Homo sapiens (human) | PGD receptor,PGD2 receptor,Prostanoid DP receptor |
| 5 | E (N) | Nanobody35|Lama glama | polymer | 139 | 15271.9 | 1 | Lama glama | ||
| 6 | F (D) | PROSTAGLANDIN D2 | non-polymer | 352.5 | 1 | Chemie (PG2) | |||
| 7 | G (D) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 8 | H (D) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 1 | Chemie (Y01) | |||
| 9 | I, J, K, L, M (A, B, C, D, N) | water | water | 18.0 | 90 | Chemie (HOH) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
D: 1 - 359 (UniProt: Q13258)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gln 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -25 | - | expression tag |
| Lys -24 | - | expression tag |
| Thr -23 | - | expression tag |
| Ile -22 | - | expression tag |
| Ile -21 | - | expression tag |
| Ala -20 | - | expression tag |
| Leu -19 | - | expression tag |
| Ser -18 | - | expression tag |
| Tyr -17 | - | expression tag |
| Ile -16 | - | expression tag |
| Phe -15 | - | expression tag |
| Cys -14 | - | expression tag |
| Leu -13 | - | expression tag |
| Val -12 | - | expression tag |
| Phe -11 | - | expression tag |
| Ala -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Tyr -8 | - | expression tag |
| Lys -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Pro 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 149838.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1060.4 | |
| All* | Total formula weight | 150899.1 |
