9E8P

Crystal Structure of GABARAP-ATG3 conjugate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ubiquitin-like-conjugating enzyme ATG3	polymer	223	25911.4	2	UniProt (Q9NT62) Pfam (PF03987)	Homo sapiens (human)	Autophagy-related protein 3,APG3-like,hApg3,Protein PC3-96
2	C, D (C, D)	Gamma-aminobutyric acid receptor-associated protein	polymer	118	14054.1	2	UniProt (O95166) Pfam (PF02991)	Homo sapiens (human)	GABA(A) receptor-associated protein,MM46
3	E (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	F, G, H, I (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
5	J, K (A, B)	water	water		18.0	9	Chemie (HOH)

Sequence modifications

A, B: 26 - 158 (UniProt: Q9NT62)

PDB	External Database	Details
Gly 25	-	expression tag

A, B: 159 - 192 (UniProt: Q9NT62)
A, B: 193 - 314 (UniProt: Q9NT62)

PDB	External Database	Details
Lys 264	Cys 264	engineered mutation

C, D: 1 - 116 (UniProt: O95166)

PDB	External Database	Details
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	79931.0
Non-Polymers*	Number of molecules	5
	Total formula weight	344.3
All*	Total formula weight	80275.4

*Water molecules are not included.