9E7U

Cryo-EM structure of NOT1:NOT8:PieF

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	Ubiquitin-like protein SMT3,CCR4-NOT transcription complex subunit 8	polymer	418	47754.7	1	UniProt (Q12306) UniProt (Q9UFF9) Pfam (PF00240) Pfam (PF04857)	Homo sapiens (human)	CAF1-like protein,CALIFp,CAF2,CCR4-associated factor 8,Caf1b
2	B (A)	Dot/Icm T4SS effector PieF	polymer	150	16568.5	1	UniProt (A0AAN5PHN6)	Legionella pneumophila
3	C (B)	CCR4-NOT transcription complex subunit 1	polymer	254	29237.8	1	UniProt (A5YKK6) Pfam (PF16415)	Homo sapiens (human)	CCR4-associated factor 1,Negative regulator of transcription subunit 1 homolog,NOT1H,hNOT1
4	D (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	E, F (C, A)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

C: -111 - -17 (UniProt: Q12306)

PDB	External Database	Details
Met -125	-	expression tag
Gly -124	-	expression tag
Ser -123	-	expression tag
Ser -122	-	expression tag
His -121	-	expression tag
His -120	-	expression tag
His -119	-	expression tag
His -118	-	expression tag
His -117	-	expression tag
His -116	-	expression tag
Ser -115	-	expression tag
Ser -114	-	expression tag
Gly -113	-	expression tag
Thr -112	-	expression tag
Thr -16	-	linker
Gly -15	-	linker
Gly -14	-	linker
Ser -13	-	linker
Thr -12	-	linker
Gly -11	-	linker
Ser -10	-	linker
Gly -9	-	linker
Leu -8	-	linker
Glu -7	-	linker
Val -6	-	linker
Leu -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Gly -2	-	linker
Pro -1	-	linker
His 0	-	linker

C: 1 - 292 (UniProt: Q9UFF9)
A: 1 - 125 (UniProt: A0AAN5PHN6)

PDB	External Database	Details
Met -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Ser -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Thr -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag

B: 1093 - 1317 (UniProt: A5YKK6)

PDB	External Database	Details
Met 1064	-	expression tag
Gly 1065	-	expression tag
Ser 1066	-	expression tag
Ser 1067	-	expression tag
His 1068	-	expression tag
His 1069	-	expression tag
His 1070	-	expression tag
His 1071	-	expression tag
His 1072	-	expression tag
His 1073	-	expression tag
Ser 1074	-	expression tag
Ser 1075	-	expression tag
Gly 1076	-	expression tag
Thr 1077	-	expression tag
Gly 1078	-	expression tag
Ser 1079	-	expression tag
Gly 1080	-	expression tag
Leu 1081	-	expression tag
Glu 1082	-	expression tag
Val 1083	-	expression tag
Leu 1084	-	expression tag
Phe 1085	-	expression tag
Gln 1086	-	expression tag
Gly 1087	-	expression tag
Pro 1088	-	expression tag
His 1089	-	expression tag
Met 1090	-	expression tag
Leu 1091	-	expression tag
Glu 1092	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	93561.1
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	93585.4

*Water molecules are not included.