9E51

Cryo-EM structure of human LPHN2 (ADGRL2)/G13 complex in lipid nanodiscs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Isoform 2 of Guanine nucleotide-binding protein subunit alpha-13	polymer	230	26574.4	1	UniProt (Q14344) Pfam (PF00503)	Homo sapiens (human)	G alpha-13,G-protein subunit alpha-13
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (A)	Adhesion G protein-coupled receptor L2	polymer	325	36891.4	1	UniProt (O95490) Pfam (PF00002)	Homo sapiens (human)	Calcium-independent alpha-latrotoxin receptor 2,CIRL-2,Latrophilin homolog 1,Latrophilin-2,Lectomedin-1
5	E, F (A)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
6	G (A)	water	water		18.0	2	Chemie (HOH)

Sequence modifications

B: 66 - 230 (UniProt: Q14344)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Thr 4	-	expression tag
Val 5	-	expression tag
Ser 6	-	expression tag
Ala 7	-	expression tag
Glu 8	-	expression tag
Asp 9	-	expression tag
Lys 10	-	expression tag
Ala 11	-	expression tag
Ala 12	-	expression tag
Ala 13	-	expression tag
Glu 14	-	expression tag
Arg 15	-	expression tag
Ser 16	-	expression tag
Lys 17	-	expression tag
Glu 18	-	expression tag
Ile 19	-	expression tag
Asp 20	-	expression tag
Lys 21	-	expression tag
Cys 22	-	expression tag
Leu 23	-	expression tag
Ser 24	-	expression tag
Arg 25	-	expression tag
Glu 26	-	expression tag
Lys 27	-	expression tag
Thr 28	-	expression tag
Tyr 29	-	expression tag
Val 30	-	expression tag
Lys 31	-	expression tag
Arg 32	-	expression tag
Leu 33	-	expression tag
Val 34	-	expression tag
Lys 35	-	expression tag
Ile 36	-	expression tag
Leu 37	-	expression tag
Leu 38	-	expression tag
Leu 39	-	expression tag
Gly 40	-	expression tag
Ala 41	-	expression tag
Asp 42	-	expression tag
Asn 43	-	expression tag
Ser 44	-	expression tag
Gly 45	-	expression tag
Lys 46	-	expression tag
Ser 47	-	expression tag
Thr 48	-	expression tag
Phe 49	-	expression tag
Leu 50	-	expression tag
Lys 51	-	expression tag
Gln 52	-	expression tag
Met 53	-	expression tag
Arg 54	-	expression tag
Ile 55	-	expression tag
Ile 56	-	expression tag
His 57	-	expression tag
Gly 58	-	expression tag
Gly 59	-	expression tag
Ser 60	-	expression tag
Gly 61	-	expression tag
Gly 62	-	expression tag
Ser 63	-	expression tag
Gly 64	-	expression tag
Gly 65	-	expression tag
Asp 111	Ser 153	conflict
Asp 114	Glu 156	conflict
-	Asp 158	deletion
-	Gln 159	deletion
-	Val 160	deletion
-	Leu 161	deletion
-	Met 162	deletion
-	Glu 163	deletion
-	Asp 164	deletion
-	Arg 165	deletion
-	Leu 166	deletion
-	Thr 167	deletion
Asp 124	Ile 176	conflict
Ala 208	Ile 260	conflict
Ile 211	Val 263	conflict

A: 825 - 1125 (UniProt: O95490)

PDB	External Database	Details
Met 824	-	initiating methionine
Gly 1126	-	expression tag
Ser 1127	-	expression tag
Leu 1128	-	expression tag
Glu 1129	-	expression tag
Val 1130	-	expression tag
Leu 1131	-	expression tag
Phe 1132	-	expression tag
Gln 1133	-	expression tag
Gly 1134	-	expression tag
Pro 1135	-	expression tag
Gly 1136	-	expression tag
Ser 1137	-	expression tag
Gly 1138	-	expression tag
Ser 1139	-	expression tag
Gly 1140	-	expression tag
Trp 1141	-	expression tag
Ser 1142	-	expression tag
His 1143	-	expression tag
Pro 1144	-	expression tag
Gln 1145	-	expression tag
Phe 1146	-	expression tag
Glu 1147	-	expression tag
Lys 1148	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	108743.9
Non-Polymers*	Number of molecules	2
	Total formula weight	773.3
All*	Total formula weight	109517.2

*Water molecules are not included.