9E3U

Cryo-EM structure of DNMT 3A2/3B3 tetramer bound to a di-nucleosome with a 25 base-pair linker

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15303.9	2	UniProt (Q71DI3) Pfam (PF00125)	Homo sapiens (human)	H3-clustered histone 13,H3-clustered histone 14,H3-clustered histone 15,Histone H3/m,Histone H3/o
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (human)
3	C, G (C, G)	Histone H2A	polymer	129	13978.2	2		Homo sapiens
4	D, H (D, H)	Histone H2B	polymer	123	13655.9	2	UniProt (A0A1B8Y854)	Homo sapiens (human)
5	I, L, M (L, V, Z)	Isoform 3 of DNA (cytosine-5)-methyltransferase 3B	polymer	773	86702.4	3	UniProt (Q9UBC3) Pfam (PF00855) Pfam (PF17980) Pfam (PF21255) Pfam (PF00145)	Homo sapiens (human)	Dnmt3b,DNA methyltransferase HsaIIIB,DNA MTase HsaIIIB,M.HsaIIIB
6	J (I)	DNA (165-MER)	polymer	165	51144.5	1		Homo sapiens
7	K (J)	DNA (165-MER)	polymer	165	50735.3	1		Homo sapiens
8	N, O (U, Y)	DNA (cytosine-5)-methyltransferase 3A	polymer	689	77914.7	2	UniProt (Q9Y6K1) Pfam (PF00145) Pfam (PF00855) Pfam (PF17980) Pfam (PF21255)	Homo sapiens (human)	Dnmt3a,DNA methyltransferase HsaIIIA,M.HsaIIIA
9	P, Q, R, S, T... (V, U, Y)	ZINC ION	non-polymer		65.4	9	Chemie (ZN)
10	V, Z (U, Y)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)

Sequence modifications

A, E: 1 - 135 (UniProt: Q71DI3)

PDB	External Database	Details
Ala 102	Gly 103	conflict

D, H: 1 - 122 (UniProt: A0A1B8Y854)

PDB	External Database	Details
Met 0	-	initiating methionine

L, V, Z: -2 - 770 (UniProt: Q9UBC3)

PDB	External Database	Details
Lys 391	-	insertion
Asp 392	-	insertion
Tyr 393	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	626481.5
Non-Polymers*	Number of molecules	11
	Total formula weight	1357.5
All*	Total formula weight	627839.0

*Water molecules are not included.