9E2T

Structure of a de novo designed interleukin-21 mimetic complex with IL-21R and IL-2Rg

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, I (A, D, G, I)	21h10	polymer	102	12186.1	4		synthetic construct
2	B, E, H, J (B, E, H, J)	Interleukin-21 receptor	polymer	212	24405.1	4	UniProt (Q9HBE5)	Homo sapiens (human)	IL-21 receptor,IL-21R,Novel interleukin receptor
3	C, F (C, F)	Cytokine receptor common subunit gamma	polymer	203	24295.1	2	UniProt (P31785) Pfam (PF22012) Pfam (PF21605)	Homo sapiens (human)	Interleukin-2 receptor subunit gamma,IL-2 receptor subunit gamma,IL-2R subunit gamma,IL-2RG,gammaC,p64
4	K, N, O, P, T... (B, C, E, F, H...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	9	Chemie (NAG)
5	AA, BA, L, M, Q... (J, B, C, E, H)	1,2-ETHANEDIOL	non-polymer		62.1	9	Chemie (EDO)
6	CA, DA, EA, FA, GA... (B, C, D, E, F...)	water	water		18.0	31	Chemie (HOH)

Sequence modifications

B, E, H, J: 1 - 209 (UniProt: Q9HBE5)

PDB	External Database	Details
Ala -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Gln 78	Asn 97	engineered mutation
Gln 85	Asn 104	engineered mutation
Asp 106	Asn 125	engineered mutation
Gln 116	Asn 135	engineered mutation

C, F: 55 - 254 (UniProt: P31785)

PDB	External Database	Details
Ala 52	-	expression tag
Asp 53	-	expression tag
Pro 54	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	194955.2
Non-Polymers*	Number of molecules	18
	Total formula weight	2549.5
All*	Total formula weight	197504.7

*Water molecules are not included.