9E2D

Cryo-EM structure of human DNMT3A2-DNMT3B3 complex bound to a di-nucleosome with a five base pair linker

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M (A, E, M, Q)	Histone H3.2	polymer	135	15303.9	4	UniProt (Q71DI3) Pfam (PF00125)	Homo sapiens (human)	H3-clustered histone 13,H3-clustered histone 14,H3-clustered histone 15,Histone H3/m,Histone H3/o
2	B, F, J, N (B, F, N, R)	Histone H4	polymer	103	11394.4	4	UniProt (P62805) Pfam (PF15511)	Homo sapiens (human)
3	C, G, K, O (C, G, O, S)	Histone H2A	polymer	129	13978.2	4		Homo sapiens
4	D, H, L, P (D, H, P, T)	Histone H2B	polymer	123	13655.9	4		Homo sapiens
5	Q, R, U, V (V, Z, L, X)	Isoform 3 of DNA (cytosine-5)-methyltransferase 3B	polymer	773	86702.4	4	UniProt (Q9UBC3) Pfam (PF00145) Pfam (PF00855) Pfam (PF17980) Pfam (PF21255)	Homo sapiens (human)	Dnmt3b,DNA methyltransferase HsaIIIB,DNA MTase HsaIIIB,M.HsaIIIB
6	S, T, W, X (U, Y, W, K)	DNA (cytosine-5)-methyltransferase 3A	polymer	689	77914.7	4	UniProt (Q9Y6K1) Pfam (PF00855) Pfam (PF17980) Pfam (PF21255) Pfam (PF00145)	Homo sapiens (human)	Dnmt3a,DNA methyltransferase HsaIIIA,M.HsaIIIA
7	Y (I)	DNA (317-MER)	polymer	317	98342.4	1		Homo sapiens
8	Z (J)	DNA (316-MER)	polymer	316	97161.7	1		Homo sapiens
9	AA, BA, CA, DA, EA... (V, U, Y, L, W...)	ZINC ION	non-polymer		65.4	18	Chemie (ZN)
10	GA, KA, RA, VA (U, Y, W, K)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	4	Chemie (SAH)

Sequence modifications

A, E, M, Q: 1 - 135 (UniProt: Q71DI3)

PDB	External Database	Details
Ala 102	Gly 103	conflict

V, Z, L, X: -2 - 770 (UniProt: Q9UBC3)

PDB	External Database	Details
Lys 391	-	insertion
Asp 392	-	insertion
Tyr 393	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	26
	Total formula weight	1071302.6
Non-Polymers*	Number of molecules	22
	Total formula weight	2715.0
All*	Total formula weight	1074017.6

*Water molecules are not included.