9E2C

Crystal structure of DEAD-box RNA helicase DDX3X R326H mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Isoform 2 of ATP-dependent RNA helicase DDX3X	polymer	498	56072.4	1	UniProt (O00571)	Homo sapiens (human)	CAP-Rf,DEAD box protein 3,X-chromosomal,DEAD box,X isoform,DBX,Helicase-like protein 2,HLP2
2	B (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
3	C (A)	water	water		18.0	71	Chemie (HOH)

Sequence modifications

A: 131 - 607 (UniProt: O00571)

PDB	External Database	Details
Met 110	-	initiating methionine
Gly 111	-	expression tag
Ser 112	-	expression tag
Ser 113	-	expression tag
His 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag
His 117	-	expression tag
His 118	-	expression tag
His 119	-	expression tag
Ser 120	-	expression tag
Ser 121	-	expression tag
Gly 122	-	expression tag
Leu 123	-	expression tag
Val 124	-	expression tag
Pro 125	-	expression tag
Arg 126	-	expression tag
Gly 127	-	expression tag
Ser 128	-	expression tag
His 129	-	expression tag
Met 130	-	expression tag
His 326	Arg 310	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	56072.4
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	56164.5

*Water molecules are not included.