9E2C

Crystal structure of DEAD-box RNA helicase DDX3X R326H mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS SIRIUS BEAMLINE MANACA
Synchrotron site	LNLS SIRIUS
Beamline	MANACA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-10
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97718
Spacegroup name	P 21 21 21
Unit cell lengths	58.347, 99.101, 105.351
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.510 - 2.300
R-factor	0.214
Rwork	0.212
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.492
Data reduction software	XDS (BUILT=20210323)
Data scaling software	XDS (BUILT=20210323)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.510	2.382
High resolution limit [Å]	2.300	2.300
Rmeas	0.114	2.273
Number of reflections	27821	2700
<I/σ(I)>	15.61	1.14
Completeness [%]	99.8	99.22
Redundancy	13	13.3
CC(1/2)	0.999	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291.15	0.1 M Bis-Tris pH 5.5, 25% PEG3350, 0.2 M Lithium sulfate, 0.01 M Strontium(II) chloride