9DVU
CryoEM structure of RpaA bound to PkaiBC DNA and Syn7942 RNAP-SigA holoenzyme
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (1) | Non-template DNA | polymer | 105 | 32396.7 | 1 | synthetic construct | ||
| 2 | B (2) | Template DNA | polymer | 105 | 32384.8 | 1 | synthetic construct | ||
| 3 | C, D (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 309 | 33814.9 | 2 | UniProt (Q31L30) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Synechococcus elongatus | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 4 | E (C) | DNA-directed RNA polymerase subunit beta | polymer | 1100 | 123428.5 | 1 | UniProt (Q31N17) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Synechococcus elongatus | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 5 | F (D) | DNA-directed RNA polymerase subunit gamma | polymer | 624 | 71054.4 | 1 | UniProt (P42079) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) | Synechococcus elongatus | RNAP subunit gamma,RNA polymerase subunit gamma,Transcriptase subunit gamma |
| 6 | G (E) | DNA-directed RNA polymerase subunit omega | polymer | 76 | 8788.0 | 1 | UniProt (Q31MH9) Pfam (PF01192) | Synechococcus elongatus | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 7 | H (F) | DNA-directed RNA polymerase subunit beta' | polymer | 1318 | 144006.0 | 1 | UniProt (Q31N15) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Synechococcus elongatus | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 8 | I (G) | RNA polymerase sigma factor SigA1 | polymer | 399 | 45776.2 | 1 | UniProt (P38023) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Synechococcus elongatus PCC 7942 = FACHB-805 | |
| 9 | J, K (R, S) | DNA-binding dual master transcriptional regulator RpaA | polymer | 249 | 28582.7 | 2 | UniProt (Q31S42) Pfam (PF00072) Pfam (PF00486) | Synechococcus elongatus PCC 7942 = FACHB-805 | Regulator of phycobilisome-associated protein A,RpaA |
| 10 | L (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 11 | M, N (D, F) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
R, S: 1 - 249 (UniProt: Q31S42)
| PDB | External Database | Details |
|---|---|---|
| Gln 121 | Arg 121 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 11 |
| Total formula weight | 582629.9 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 582785.0 |
