9DSO

CRYO-EM STRUCTURE OF SACCHAROMYCES CEREVISIAE RAT1-RAI1-RTT103 COMPLEX

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	5'-3' exoribonuclease 2	polymer	989	114204.0	1	UniProt (Q02792) Pfam (PF03159) Pfam (PF17846)	Saccharomyces cerevisiae (brewer's yeast)	Ribonucleic acid-trafficking protein 1,p116
2	B (B)	Decapping nuclease RAI1	polymer	387	44571.4	1	UniProt (P53063) Pfam (PF08652)	Saccharomyces cerevisiae (brewer's yeast)	ScRai1,NAD-capped RNA hydrolase RAI1,DeNADding enzyme RAI1,RAT1-interacting protein
3	C (C)	Regulator of Ty1 transposition protein 103	polymer	429	48725.5	1	UniProt (Q05543) Pfam (PF04818)	Saccharomyces cerevisiae (brewer's yeast)
4	D (B)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A: 1 - 981 (UniProt: Q02792)

PDB	External Database	Details
Lys 974	Asn 974	conflict
Leu 982	-	expression tag
Glu 983	-	expression tag
His 984	-	expression tag
His 985	-	expression tag
His 986	-	expression tag
His 987	-	expression tag
His 988	-	expression tag
His 989	-	expression tag

C: 1 - 409 (UniProt: Q05543)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	207500.9
Non-Polymers*	Number of molecules	1
	Total formula weight	24.3
All*	Total formula weight	207525.3

*Water molecules are not included.