9DFW
X-ray crystal structure of an engineered Viperin-like enzyme from T. virens with bound CTP and SAM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Radical SAM core domain-containing protein | polymer | 317 | 36369.1 | 3 | UniProt (G9MQB8) Pfam (PF13353) Pfam (PF04055) | Trichoderma virens | |
| 2 | D, K, N (A, B, C) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 3 | Chemie (SF4) | |||
| 3 | E, L, O (A, B, C) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 3 | Chemie (SAM) | |||
| 4 | F, M, P (A, B, C) | CYTIDINE-5'-TRIPHOSPHATE | non-polymer | 483.2 | 3 | Chemie (CTP) | |||
| 5 | G, H (A) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 2 | Chemie (EPE) | |||
| 6 | I, J, R (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 7 | Q (C) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 8 | S, T, U (A, B, C) | water | water | 18.0 | 710 | Chemie (HOH) |
Sequence modifications
A, B, C: 18 - 311 (UniProt: G9MQB8)
| PDB | External Database | Details |
|---|---|---|
| Met -5 | - | initiating methionine |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| Ser 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Gly 4 | - | expression tag |
| Val 5 | - | expression tag |
| Asp 6 | - | expression tag |
| Leu 7 | - | expression tag |
| Gly 8 | - | expression tag |
| Thr 9 | - | expression tag |
| Glu 10 | - | expression tag |
| Asn 11 | - | expression tag |
| Leu 12 | - | expression tag |
| Tyr 13 | - | expression tag |
| Phe 14 | - | expression tag |
| Gln 15 | - | expression tag |
| Ser 16 | - | expression tag |
| Met 17 | - | expression tag |
| Ile 198 | Val 197 | engineered mutation |
| Glu 199 | Thr 198 | engineered mutation |
| Asn 257 | Asp 256 | engineered mutation |
| Cys 258 | Arg 257 | engineered mutation |
| Thr 259 | Asn 258 | engineered mutation |
| Gly 261 | - | insertion |
| Arg 262 | - | insertion |
| Lys 263 | Gln 260 | engineered mutation |
| Asp 264 | Gln 261 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 109107.3 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 4341.3 | |
| All* | Total formula weight | 113448.7 |
