9DFW
X-ray crystal structure of an engineered Viperin-like enzyme from T. virens with bound CTP and SAM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Radical SAM core domain-containing protein | polymer | 317 | 36369.1 | 3 | UniProt (G9MQB8) Pfam (PF13353) Pfam (PF04055) | Trichoderma virens | |
| 2 | D, K, N (A, B, C) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 3 | Chemie (SF4) | |||
| 3 | E, L, O (A, B, C) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 3 | Chemie (SAM) PubChem (5136) PubChem (34755) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (9865603) PubChem (145712080) PubChem (12312798) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094) | |||
| 4 | F, M, P (A, B, C) | CYTIDINE-5'-TRIPHOSPHATE | non-polymer | 483.2 | 3 | Chemie (CTP) | |||
| 5 | G, H (A) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 2 | Chemie (EPE) PubChem (23831) | |||
| 6 | I, J, R (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) PubChem (888) | |||
| 7 | Q (C) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) PubChem (753) | |||
| 8 | S, T, U (A, B, C) | water | water | 18.0 | 710 | Chemie (HOH) |
Sequence modifications
A, B, C: 18 - 311 (UniProt: G9MQB8)
| PDB | External Database | Details |
|---|---|---|
| Met -5 | - | initiating methionine |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| Ser 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Gly 4 | - | expression tag |
| Val 5 | - | expression tag |
| Asp 6 | - | expression tag |
| Leu 7 | - | expression tag |
| Gly 8 | - | expression tag |
| Thr 9 | - | expression tag |
| Glu 10 | - | expression tag |
| Asn 11 | - | expression tag |
| Leu 12 | - | expression tag |
| Tyr 13 | - | expression tag |
| Phe 14 | - | expression tag |
| Gln 15 | - | expression tag |
| Ser 16 | - | expression tag |
| Met 17 | - | expression tag |
| Ile 198 | Val 197 | engineered mutation |
| Glu 199 | Thr 198 | engineered mutation |
| Asn 257 | Asp 256 | engineered mutation |
| Cys 258 | Arg 257 | engineered mutation |
| Thr 259 | Asn 258 | engineered mutation |
| Gly 261 | - | insertion |
| Arg 262 | - | insertion |
| Lys 263 | Gln 260 | engineered mutation |
| Asp 264 | Gln 261 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 109107.3 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 4341.3 | |
| All* | Total formula weight | 113448.7 |
