9D2B

Symmetry-expanded reconstruction of augmin T-II bonsai on the microtubule

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (C, A)	Tubulin alpha-1B chain	polymer	451	50204.4	2	UniProt (P81947) Pfam (PF00091) Pfam (PF03953)	Bos taurus (domestic cattle)	Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain
2	B (B)	Tubulin beta-2B chain	polymer	445	49999.9	1	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953)	Bos taurus (domestic cattle)
3	D (F)	HAUS augmin like complex subunit 6 L homeolog isoform X1	polymer	289	33562.7	1	UniProt (A0A8J1MAE8) Pfam (PF14661)	Xenopus laevis (African clawed frog)
4	E (G)	HAUS augmin like complex subunit 7 S homeolog	polymer	282	31848.1	1	UniProt (B1H1T5)	Xenopus laevis (African clawed frog)	LOC100158301 protein
5	F (H)	HAUS augmin-like complex subunit 8	polymer	260	28742.9	1	UniProt (Q0IHJ3)	Xenopus laevis (African clawed frog)	HEC1/NDC80-interacting centrosome-associated protein 1,Sarcoma antigen NY-SAR-48 homolog
6	G, K (C, A)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
7	H, J, L (C, B, A)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
8	I (B)	PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER	non-polymer		521.2	1	Chemie (G2P)

Sequence modifications

F: 1 - 268 (UniProt: A0A8J1MAE8)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Arg -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
His 8	Gln 8	conflict
Val 70	Met 70	conflict
Leu 198	-	insertion
Leu 199	-	insertion
Ile 200	-	insertion
Lys 201	-	insertion
Gln 202	-	insertion
Ile 203	-	insertion
Arg 204	-	insertion
Asp 205	-	insertion
Met 206	-	insertion
Arg 207	-	insertion
Ser 208	-	insertion
Glu 209	-	insertion
His 210	-	insertion
Val 211	-	insertion
Ala 212	-	insertion
Leu 213	-	insertion
Gln 214	-	insertion
Asn 215	-	insertion
Gln 216	-	insertion
Gln 217	-	insertion
Lys 218	-	insertion

G: 2 - 261 (UniProt: B1H1T5)

PDB	External Database	Details
Met -12	-	expression tag
Ala -11	-	expression tag
Asp -10	-	expression tag
Pro -9	-	expression tag
Trp -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
Pro -5	-	expression tag
Gln -4	-	expression tag
Phe -3	-	expression tag
Glu -2	-	expression tag
Lys -1	-	expression tag
Gly 0	-	expression tag
Gly 1	-	expression tag
Trp 262	-	expression tag
Ser 263	-	expression tag
His 264	-	expression tag
Pro 265	-	expression tag
Gln 266	-	expression tag
Phe 267	-	expression tag
Glu 268	-	expression tag
Lys 269	-	expression tag

H: 1 - 257 (UniProt: Q0IHJ3)

PDB	External Database	Details
Met -2	-	expression tag
Arg -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	244562.5
Non-Polymers*	Number of molecules	6
	Total formula weight	1640.5
All*	Total formula weight	246203.0

*Water molecules are not included.