9D2B
Symmetry-expanded reconstruction of augmin T-II bonsai on the microtubule
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.1 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.08 |
| Number of Particle | 177242 |
| Software | cryoSPARC,FREALIGN,Coot,PHENIX,EPU,CTFFIND,UCSF Chimera |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
