9D0A
CryoEM structure of PAR2 with endogenous tethered ligand.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 339 | 37285.7 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 2 | B (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 59 | 6504.4 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 3 | C (E) | scFv16 | polymer | 251 | 26679.7 | 1 | unidentified | ||
| 4 | D (R) | Proteinase-activated receptor 2 | polymer | 397 | 44173.2 | 1 | UniProt (P55085) Pfam (PF00001) | Homo sapiens (human) | PAR-2,Coagulation factor II receptor-like 1,G-protein coupled receptor 11,Thrombin receptor-like 1 |
| 5 | E (A) | Guanine nucleotide-binding protein G(q) subunit alpha, Guanine nucleotide-binding protein G(s) subunit alpha isoforms short chimera,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 360 | 41972.6 | 1 | UniProt (P50148) UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Guanine nucleotide-binding protein alpha-q,Adenylate cyclase-stimulating G alpha protein |
Sequence modifications
A: 7 - 197 (UniProt: P50148)
A: 198 - 366 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Gly 8 | Ala 8 | conflict |
| Thr 10 | Cys 10 | conflict |
| Ala 13 | Glu 13 | conflict |
| Asp 15 | Ala 15 | conflict |
| Ala 17 | Glu 17 | conflict |
| Val 19 | Arg 19 | conflict |
| Glu 20 | Arg 20 | conflict |
| Arg 21 | Ile 21 | conflict |
| Ser 22 | Asn 22 | conflict |
| Lys 23 | Asp 23 | conflict |
| Met 24 | Glu 24 | conflict |
| Asp 26 | Glu 26 | conflict |
| Asn 28 | Gln 28 | conflict |
| Glu 31 | Arg 31 | conflict |
| Gly 33 | Lys 33 | conflict |
| Glu 34 | Arg 34 | conflict |
| Lys 35 | Asp 35 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ser 211 | Asp 229 | conflict |
| Asp 231 | Ala 249 | conflict |
| Asp 234 | Ser 252 | conflict |
| - | Asn 254 | deletion |
| - | Met 255 | deletion |
| - | Val 256 | deletion |
| - | Ile 257 | deletion |
| - | Arg 258 | deletion |
| - | Glu 259 | deletion |
| - | Asp 260 | deletion |
| - | Asn 261 | deletion |
| - | Gln 262 | deletion |
| - | Thr 263 | deletion |
| Asp 244 | Leu 272 | conflict |
| Lys 315 | Asp 343 | conflict |
| Val 318 | Leu 346 | conflict |
| Asp 319 | Arg 347 | conflict |
| Ile 330 | Tyr 358 | conflict |
| Ala 344 | Ile 372 | conflict |
| Ile 347 | Val 375 | conflict |
| Lys 352 | Arg 380 | conflict |
| Leu 356 | Gln 384 | conflict |
| Gln 357 | Arg 385 | conflict |
| Asn 359 | His 387 | conflict |
| Glu 362 | Gln 390 | conflict |
| Asn 364 | Glu 392 | conflict |
| Val 366 | Leu 394 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 156615.7 | |
| All* | Total formula weight | 156615.7 |
