9CIK
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23R/L36D at cryogenic temperature
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Thermonuclease | polymer | 143 | 16203.5 | 1 | UniProt (Q5HHM4) Pfam (PF00565) | Staphylococcus aureus | TNase,Micrococcal nuclease,Staphylococcal nuclease |
| 2 | B (A) | THYMIDINE-3',5'-DIPHOSPHATE | non-polymer | 402.2 | 1 | Chemie (THP) | |||
| 3 | C (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 4 | D (A) | water | water | 18.0 | 103 | Chemie (HOH) |
Sequence modifications
A: 1 - 149 (UniProt: Q5HHM4)
| PDB | External Database | Details |
|---|---|---|
| Arg 23 | Val 102 | engineered mutation |
| Asp 36 | Leu 115 | engineered mutation |
| Phe 50 | Thr 123 | conflict |
| Asn 51 | Lys 124 | conflict |
| - | His 125 | deletion |
| - | Pro 126 | deletion |
| - | Lys 127 | deletion |
| - | Lys 128 | deletion |
| - | Gly 129 | deletion |
| - | Val 130 | deletion |
| Gly 117 | Pro 196 | conflict |
| Ala 128 | Ser 207 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 16203.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 442.3 | |
| All* | Total formula weight | 16645.7 |
