9CIK
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23R/L36D at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | Agilent SuperNova |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2022-12-15 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.875, 60.382, 38.196 |
| Unit cell angles | 90.00, 94.06, 90.00 |
Refinement procedure
| Resolution | 19.180 - 1.950 |
| R-factor | 0.17308 |
| Rwork | 0.171 |
| R-free | 0.20829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.776 |
| Data reduction software | CrysalisPro (42.49 64-bit (release 15-03-2022)) |
| Data scaling software | CrysalisPro (42.49 64-bit (release 15-03-2022)) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.110 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.016 | 0.148 |
| Number of reflections | 10282 | 1030 |
| <I/σ(I)> | 53.8 | 5.85 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 28% MPD, 25 mM Potassium Phosphate |
