9CHT

Human E3 ligase E6AP in complex with HPV16-E6 and p53

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin-protein ligase E3A	polymer	895	102982.9	1	UniProt (Q05086) Pfam (PF16558) Pfam (PF00632)	Homo sapiens (human)	E6AP ubiquitin-protein ligase,HECT-type ubiquitin transferase E3A,Human papillomavirus E6-associated protein,Oncogenic protein-associated protein E6-AP,Renal carcinoma antigen NY-REN-54
2	B (B)	Immunoglobulin G-binding protein G/Cellular tumor antigen p53 fusion protein	polymer	396	43801.7	1	UniProt (P06654) UniProt (P04637) Pfam (PF08563) Pfam (PF18521) Pfam (PF00870)	Streptococcus sp. group G
3	C (C)	Protein E6	polymer	151	18365.4	1	UniProt (P03126) Pfam (PF00518)	Human papillomavirus 16

Sequence modifications

A: 1 - 875 (UniProt: Q05086)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

B: -71 - -17 (UniProt: P06654)

PDB	External Database	Details
Met -83	-	initiating methionine
Gly -82	-	expression tag
His -81	-	expression tag
His -80	-	expression tag
His -79	-	expression tag
His -78	-	expression tag
His -77	-	expression tag
His -76	-	expression tag
Ser -75	-	expression tag
Ser -74	-	expression tag
Gly -73	-	expression tag
Met -72	-	expression tag
Glu -16	-	linker
Phe -15	-	linker
Ser -14	-	linker
Ser -13	-	linker
Gly -12	-	linker
Ser -11	-	linker
Ser -10	-	linker
Gly -9	-	linker
Glu -8	-	linker
Asn -7	-	linker
Leu -6	-	linker
Tyr -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Gly -2	-	linker
Ser -1	-	linker
His 0	-	linker

B: 1 - 312 (UniProt: P04637)

PDB	External Database	Details
Leu 133	Met 133	conflict
Ala 203	Val 203	conflict
Tyr 239	Asn 239	conflict
Asp 268	Asn 268	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	165150.0
All*	Total formula weight	165150.0

*Water molecules are not included.