9CCA

Cryo-EM structure of a designed pyridoxal phosphate (PLP) synthase fused to a designed circumsporozoite protein antigen from Plasmodium falciparum (CSP-P1-CSP and CSP-P2-CSP)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Circumsporozoite protein,Pyridoxal 5'-phosphate synthase subunit Pdx1	polymer	537	58417.1	12	UniProt (Q7K740) UniProt (C6KT50) Pfam (PF01680) Pfam (PF00090)	Plasmodium falciparum (malaria parasite P. falciparum)
2	G, H, I, J, K... (G, H, I, J, K...)	Circumsporozoite protein,Pyridoxal 5'-phosphate synthase subunit PDX2	polymer	454	49843.6	12	UniProt (Q7K740) UniProt (Q8IIK4) Pfam (PF01174) Pfam (PF00090)	Plasmodium falciparum (malaria parasite P. falciparum)

Sequence modifications

A, B, C, D, E, F, M, N, O, P, Q, R: 4 - 17 (UniProt: Q7K740)

PDB	External Database	Details
Met 1	-	initiating methionine
Thr 2	-	cloning artifact
Gly 3	-	cloning artifact

A, B, C, D, E, F, M, N, O, P, Q, R: 419 - 432 (UniProt: Q7K740)

PDB	External Database	Details
Gly 112	-	linker
Gly 113	-	linker
Ser 114	-	linker
Gly 115	-	cloning artifact
Thr 116	-	cloning artifact

A, B, C, D, E, F, M, N, O, P, Q, R: 18 - 37 (UniProt: Q7K740)
A, B, C, D, E, F, M, N, O, P, Q, R: 433 - 452 (UniProt: Q7K740)
A, B, C, D, E, F, M, N, O, P, Q, R: 38 - 111 (UniProt: Q7K740)
A, B, C, D, E, F, M, N, O, P, Q, R: 453 - 526 (UniProt: Q7K740)

PDB	External Database	Details
Gly 527	-	linker
Gly 528	-	linker
Ser 529	-	linker
Leu 530	-	cloning artifact
Glu 531	-	cloning artifact
His 532	-	expression tag
His 533	-	expression tag
His 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag
His 537	-	expression tag

A, B, C, D, E, F, M, N, O, P, Q, R: 117 - 416 (UniProt: C6KT50)

PDB	External Database	Details
Arg 198	Lys 83	engineered mutation
Cys 289	Lys 174	engineered mutation
Cys 293	Ser 178	engineered mutation
Thr 417	-	cloning artifact
Gly 418	-	cloning artifact

G, H, I, J, K, L, S, T, U, V, W, X: 4 - 17 (UniProt: Q7K740)

PDB	External Database	Details
Met 1	-	initiating methionine
Thr 2	-	cloning artifact
Gly 3	-	cloning artifact

G, H, I, J, K, L, S, T, U, V, W, X: 336 - 349 (UniProt: Q7K740)

PDB	External Database	Details
Gly 112	-	linker
Gly 113	-	linker
Ser 114	-	linker
Gly 115	-	cloning artifact
Thr 116	-	cloning artifact

G, H, I, J, K, L, S, T, U, V, W, X: 18 - 37 (UniProt: Q7K740)
G, H, I, J, K, L, S, T, U, V, W, X: 350 - 369 (UniProt: Q7K740)
G, H, I, J, K, L, S, T, U, V, W, X: 38 - 111 (UniProt: Q7K740)
G, H, I, J, K, L, S, T, U, V, W, X: 370 - 443 (UniProt: Q7K740)

PDB	External Database	Details
Gly 444	-	linker
Gly 445	-	linker
Ser 446	-	linker
Leu 447	-	cloning artifact
Glu 448	-	cloning artifact
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag
His 453	-	expression tag
His 454	-	expression tag

G, H, I, J, K, L, S, T, U, V, W, X: 117 - 333 (UniProt: Q8IIK4)

PDB	External Database	Details
Gln 179	Asn 65	engineered mutation
Gln 242	Asn 128	engineered mutation
Asn 310	His 196	engineered mutation
Thr 334	-	cloning artifact
Gly 335	-	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	1299128.1
All*	Total formula weight	1299128.1

*Water molecules are not included.