9CCA

Cryo-EM structure of a designed pyridoxal phosphate (PLP) synthase fused to a designed circumsporozoite protein antigen from Plasmodium falciparum (CSP-P1-CSP and CSP-P2-CSP)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000304	biological_process	response to singlet oxygen
A	0003824	molecular_function	catalytic activity
A	0004359	molecular_function	glutaminase activity
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0008615	biological_process	pyridoxine biosynthetic process
A	0016843	molecular_function	amine-lyase activity
A	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
A	0042802	molecular_function	identical protein binding
A	0042819	biological_process	vitamin B6 biosynthetic process
A	0042823	biological_process	pyridoxal phosphate biosynthetic process
B	0000304	biological_process	response to singlet oxygen
B	0003824	molecular_function	catalytic activity
B	0004359	molecular_function	glutaminase activity
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0008615	biological_process	pyridoxine biosynthetic process
B	0016843	molecular_function	amine-lyase activity
B	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
B	0042802	molecular_function	identical protein binding
B	0042819	biological_process	vitamin B6 biosynthetic process
B	0042823	biological_process	pyridoxal phosphate biosynthetic process
C	0000304	biological_process	response to singlet oxygen
C	0003824	molecular_function	catalytic activity
C	0004359	molecular_function	glutaminase activity
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0008615	biological_process	pyridoxine biosynthetic process
C	0016843	molecular_function	amine-lyase activity
C	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
C	0042802	molecular_function	identical protein binding
C	0042819	biological_process	vitamin B6 biosynthetic process
C	0042823	biological_process	pyridoxal phosphate biosynthetic process
D	0000304	biological_process	response to singlet oxygen
D	0003824	molecular_function	catalytic activity
D	0004359	molecular_function	glutaminase activity
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0008615	biological_process	pyridoxine biosynthetic process
D	0016843	molecular_function	amine-lyase activity
D	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
D	0042802	molecular_function	identical protein binding
D	0042819	biological_process	vitamin B6 biosynthetic process
D	0042823	biological_process	pyridoxal phosphate biosynthetic process
E	0000304	biological_process	response to singlet oxygen
E	0003824	molecular_function	catalytic activity
E	0004359	molecular_function	glutaminase activity
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0008615	biological_process	pyridoxine biosynthetic process
E	0016843	molecular_function	amine-lyase activity
E	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
E	0042802	molecular_function	identical protein binding
E	0042819	biological_process	vitamin B6 biosynthetic process
E	0042823	biological_process	pyridoxal phosphate biosynthetic process
F	0000304	biological_process	response to singlet oxygen
F	0003824	molecular_function	catalytic activity
F	0004359	molecular_function	glutaminase activity
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0008615	biological_process	pyridoxine biosynthetic process
F	0016843	molecular_function	amine-lyase activity
F	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
F	0042802	molecular_function	identical protein binding
F	0042819	biological_process	vitamin B6 biosynthetic process
F	0042823	biological_process	pyridoxal phosphate biosynthetic process
G	0000304	biological_process	response to singlet oxygen
G	0004359	molecular_function	glutaminase activity
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0008614	biological_process	pyridoxine metabolic process
G	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
G	0042819	biological_process	vitamin B6 biosynthetic process
G	0042823	biological_process	pyridoxal phosphate biosynthetic process
G	1903600	cellular_component	glutaminase complex
H	0000304	biological_process	response to singlet oxygen
H	0004359	molecular_function	glutaminase activity
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0008614	biological_process	pyridoxine metabolic process
H	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
H	0042819	biological_process	vitamin B6 biosynthetic process
H	0042823	biological_process	pyridoxal phosphate biosynthetic process
H	1903600	cellular_component	glutaminase complex
I	0000304	biological_process	response to singlet oxygen
I	0004359	molecular_function	glutaminase activity
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0008614	biological_process	pyridoxine metabolic process
I	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
I	0042819	biological_process	vitamin B6 biosynthetic process
I	0042823	biological_process	pyridoxal phosphate biosynthetic process
I	1903600	cellular_component	glutaminase complex
J	0000304	biological_process	response to singlet oxygen
J	0004359	molecular_function	glutaminase activity
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0008614	biological_process	pyridoxine metabolic process
J	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
J	0042819	biological_process	vitamin B6 biosynthetic process
J	0042823	biological_process	pyridoxal phosphate biosynthetic process
J	1903600	cellular_component	glutaminase complex
K	0000304	biological_process	response to singlet oxygen
K	0004359	molecular_function	glutaminase activity
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0008614	biological_process	pyridoxine metabolic process
K	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
K	0042819	biological_process	vitamin B6 biosynthetic process
K	0042823	biological_process	pyridoxal phosphate biosynthetic process
K	1903600	cellular_component	glutaminase complex
L	0000304	biological_process	response to singlet oxygen
L	0004359	molecular_function	glutaminase activity
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0008614	biological_process	pyridoxine metabolic process
L	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
L	0042819	biological_process	vitamin B6 biosynthetic process
L	0042823	biological_process	pyridoxal phosphate biosynthetic process
L	1903600	cellular_component	glutaminase complex
M	0000304	biological_process	response to singlet oxygen
M	0003824	molecular_function	catalytic activity
M	0004359	molecular_function	glutaminase activity
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0008615	biological_process	pyridoxine biosynthetic process
M	0016843	molecular_function	amine-lyase activity
M	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
M	0042802	molecular_function	identical protein binding
M	0042819	biological_process	vitamin B6 biosynthetic process
M	0042823	biological_process	pyridoxal phosphate biosynthetic process
N	0000304	biological_process	response to singlet oxygen
N	0003824	molecular_function	catalytic activity
N	0004359	molecular_function	glutaminase activity
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0008615	biological_process	pyridoxine biosynthetic process
N	0016843	molecular_function	amine-lyase activity
N	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
N	0042802	molecular_function	identical protein binding
N	0042819	biological_process	vitamin B6 biosynthetic process
N	0042823	biological_process	pyridoxal phosphate biosynthetic process
O	0000304	biological_process	response to singlet oxygen
O	0003824	molecular_function	catalytic activity
O	0004359	molecular_function	glutaminase activity
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0008615	biological_process	pyridoxine biosynthetic process
O	0016843	molecular_function	amine-lyase activity
O	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
O	0042802	molecular_function	identical protein binding
O	0042819	biological_process	vitamin B6 biosynthetic process
O	0042823	biological_process	pyridoxal phosphate biosynthetic process
P	0000304	biological_process	response to singlet oxygen
P	0003824	molecular_function	catalytic activity
P	0004359	molecular_function	glutaminase activity
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0008615	biological_process	pyridoxine biosynthetic process
P	0016843	molecular_function	amine-lyase activity
P	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
P	0042802	molecular_function	identical protein binding
P	0042819	biological_process	vitamin B6 biosynthetic process
P	0042823	biological_process	pyridoxal phosphate biosynthetic process
Q	0000304	biological_process	response to singlet oxygen
Q	0003824	molecular_function	catalytic activity
Q	0004359	molecular_function	glutaminase activity
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0008615	biological_process	pyridoxine biosynthetic process
Q	0016843	molecular_function	amine-lyase activity
Q	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
Q	0042802	molecular_function	identical protein binding
Q	0042819	biological_process	vitamin B6 biosynthetic process
Q	0042823	biological_process	pyridoxal phosphate biosynthetic process
R	0000304	biological_process	response to singlet oxygen
R	0003824	molecular_function	catalytic activity
R	0004359	molecular_function	glutaminase activity
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0008615	biological_process	pyridoxine biosynthetic process
R	0016843	molecular_function	amine-lyase activity
R	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
R	0042802	molecular_function	identical protein binding
R	0042819	biological_process	vitamin B6 biosynthetic process
R	0042823	biological_process	pyridoxal phosphate biosynthetic process
S	0000304	biological_process	response to singlet oxygen
S	0004359	molecular_function	glutaminase activity
S	0005737	cellular_component	cytoplasm
S	0005829	cellular_component	cytosol
S	0008614	biological_process	pyridoxine metabolic process
S	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
S	0042819	biological_process	vitamin B6 biosynthetic process
S	0042823	biological_process	pyridoxal phosphate biosynthetic process
S	1903600	cellular_component	glutaminase complex
T	0000304	biological_process	response to singlet oxygen
T	0004359	molecular_function	glutaminase activity
T	0005737	cellular_component	cytoplasm
T	0005829	cellular_component	cytosol
T	0008614	biological_process	pyridoxine metabolic process
T	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
T	0042819	biological_process	vitamin B6 biosynthetic process
T	0042823	biological_process	pyridoxal phosphate biosynthetic process
T	1903600	cellular_component	glutaminase complex
U	0000304	biological_process	response to singlet oxygen
U	0004359	molecular_function	glutaminase activity
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0008614	biological_process	pyridoxine metabolic process
U	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
U	0042819	biological_process	vitamin B6 biosynthetic process
U	0042823	biological_process	pyridoxal phosphate biosynthetic process
U	1903600	cellular_component	glutaminase complex
V	0000304	biological_process	response to singlet oxygen
V	0004359	molecular_function	glutaminase activity
V	0005737	cellular_component	cytoplasm
V	0005829	cellular_component	cytosol
V	0008614	biological_process	pyridoxine metabolic process
V	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
V	0042819	biological_process	vitamin B6 biosynthetic process
V	0042823	biological_process	pyridoxal phosphate biosynthetic process
V	1903600	cellular_component	glutaminase complex
W	0000304	biological_process	response to singlet oxygen
W	0004359	molecular_function	glutaminase activity
W	0005737	cellular_component	cytoplasm
W	0005829	cellular_component	cytosol
W	0008614	biological_process	pyridoxine metabolic process
W	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
W	0042819	biological_process	vitamin B6 biosynthetic process
W	0042823	biological_process	pyridoxal phosphate biosynthetic process
W	1903600	cellular_component	glutaminase complex
X	0000304	biological_process	response to singlet oxygen
X	0004359	molecular_function	glutaminase activity
X	0005737	cellular_component	cytoplasm
X	0005829	cellular_component	cytosol
X	0008614	biological_process	pyridoxine metabolic process
X	0036381	molecular_function	pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity
X	0042819	biological_process	vitamin B6 biosynthetic process
X	0042823	biological_process	pyridoxal phosphate biosynthetic process
X	1903600	cellular_component	glutaminase complex

Functional Information from PROSITE/UniProt

site_id	PS01235
Number of Residues	19
Details	PDXS_SNZ_1 PdxS/SNZ family signature. LPVVNFAAGGVATPADAAM

Chain	Residue	Details
A	LEU322-MET340

---

site_id	PS01236
Number of Residues	11
Details	PDXT_SNO_1 PdxT/SNO family family signature. GLVIPGGESTT

Chain	Residue	Details
G	GLY160-THR170

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	Active site: {"description":"Schiff-base intermediate with D-ribose 5-phosphate","evidences":[{"source":"UniProtKB","id":"P0DMS0","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	48
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P0DMS0","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q03148","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"UniProtKB","id":"P37528","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	24
Details	Active site: {"description":"Charge relay system","evidences":[{"source":"UniProtKB","id":"P37528","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	48
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P37528","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---