9CBM

Cryo-EM structure of dexmedetomidine-bound alpha-2A-adrenergic receptor in complex with heterotrimeric Gi-protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	379	43147.1	1	UniProt (P10824) Pfam (PF00503) In PDB	Rattus norvegicus (Norway rat)	Adenylate cyclase-inhibiting G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	339	37285.7	1	UniProt (P62871) Pfam (PF00400) In PDB	Bos taurus (cattle)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (cattle)	G gamma-I
4	R	Endolysin,Alpha-2A adrenergic receptor	polymer	484	54715.9	1	UniProt (P00720) UniProt (P08913) Pfam (PF00959) Pfam (PF00001) In PDB	Enterobacteria phage T4 (Bacteriophage T4)	Lysis protein,Lysozyme,Muramidase,Alpha-2 adrenergic receptor subtype C10,Alpha-2A adrenoreceptor,Alpha-2A adrenoceptor,Alpha-2AAR
5	R	4-[(1~{S})-1-(2,3-dimethylphenyl)ethyl]-1~{H}-imidazole	non-polymer		200.3	1	Chemie (CZX)

Sequence modifications

A: 1 - 354 (UniProt: P10824)

PDB	External Database	Details
Met -24	-	initiating methionine
Gly -23	-	expression tag
Ser -22	-	expression tag
Ser -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Leu -11	-	expression tag
Glu -10	-	expression tag
Val -9	-	expression tag
Leu -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Gly -5	-	expression tag
Pro -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ala 203	Gly 203	engineered mutation

G: 1 - 71 (UniProt: P63212)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

R: -128 - 31 (UniProt: P00720)

PDB	External Database	Details
Met -152	-	initiating methionine
Lys -151	-	expression tag
Thr -150	-	expression tag
Ile -149	-	expression tag
Ile -148	-	expression tag
Ala -147	-	expression tag
Leu -146	-	expression tag
Ser -145	-	expression tag
Tyr -144	-	expression tag
Ile -143	-	expression tag
Phe -142	-	expression tag
Cys -141	-	expression tag
Leu -140	-	expression tag
Val -139	-	expression tag
Phe -138	-	expression tag
Ala -137	-	expression tag
Asp -136	-	expression tag
Tyr -135	-	expression tag
Lys -134	-	expression tag
Asp -133	-	expression tag
Asp -132	-	expression tag
Asp -131	-	expression tag
Asp -130	-	expression tag
Lys -129	-	expression tag
Gly -118	Arg 12	conflict
Thr -76	Cys 54	conflict
Ala -33	Cys 97	conflict
Arg 7	Ile 137	conflict
Ala 32	-	linker
Ala 33	-	linker
Ala 34	-	linker

R: 35 - 242 (UniProt: P08913)
R: 380 - 460 (UniProt: P08913)

PDB	External Database	Details
Ala 461	-	expression tag
Ser 462	-	expression tag
Leu 463	-	expression tag
Glu 464	-	expression tag
Val 465	-	expression tag
Leu 466	-	expression tag
Phe 467	-	expression tag
Gln 468	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	142993.8
Non-Polymers*	Number of molecules	1
	Total formula weight	200.3
All*	Total formula weight	143194.0

*Water molecules are not included.