9C5B
AP-3 bound to myristoylated Arf1 (Q71L) and LAMPI on a lipid nanodisc; combined map
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, C) | ADP-ribosylation factor 1 | polymer | 182 | 20775.8 | 2 | UniProt (P84077) Pfam (PF00025) | Homo sapiens (human) | |
| 2 | C (D) | AP-3 complex subunit delta-1 | polymer | 617 | 69382.0 | 1 | UniProt (O14617) Pfam (PF01602) | Homo sapiens (human) | AP-3 complex subunit delta,Adaptor-related protein complex 3 subunit delta-1,Delta-adaptin |
| 3 | D (M) | AP-3 complex subunit mu-1 | polymer | 418 | 46990.0 | 1 | UniProt (Q9Y2T2) Pfam (PF01217) Pfam (PF00928) | Homo sapiens (human) | AP-3 adaptor complex mu3A subunit,Adaptor-related protein complex 3 subunit mu-1,Mu-adaptin 3A,Mu3A-adaptin |
| 4 | E (S) | AP-3 complex subunit sigma-1 | polymer | 193 | 21755.1 | 1 | UniProt (Q92572) Pfam (PF01217) | Homo sapiens (human) | AP-3 complex subunit sigma-3A,Adaptor-related protein complex 3 subunit sigma-1,Clathrin-associated/assembly/adaptor protein,small 3,Sigma-3A-adaptin,Sigma3A-adaptin,Sigma-adaptin 3a |
| 5 | F (Y) | Lysosome-associated membrane glycoprotein 1 | polymer | 12 | 1377.6 | 1 | UniProt (P11279) | Homo sapiens | LAMP-1,Lysosome-associated membrane protein 1,CD107 antigen-like family member A |
| 6 | G (B) | AP-3 complex subunit beta-1 | polymer | 677 | 76499.6 | 1 | UniProt (O00203) Pfam (PF01602) | Homo sapiens (human) | Adaptor protein complex AP-3 subunit beta-1,Adaptor-related protein complex 3 subunit beta-1,Beta-3A-adaptin,Clathrin assembly protein complex 3 beta-1 large chain |
| 7 | H, J (A, C) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 8 | I, K (A, C) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 2 | Chemie (GTP) |
Sequence modifications
A, C: 2 - 181 (UniProt: P84077)
| PDB | External Database | Details |
|---|---|---|
| Leu 71 | Gln 71 | engineered mutation |
| Ser 182 | - | expression tag |
| Leu 183 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 257555.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1095.0 | |
| All* | Total formula weight | 258650.8 |
