9C59
Human AP-3 dimer bound to myristoylated Arf1 (Q71L) and LAMP1 cargo on a lipid nanodisc
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, H (D, d) | AP-3 complex subunit delta-1 | polymer | 617 | 69382.0 | 2 | UniProt (O14617) Pfam (PF01602) | Homo sapiens (human) | AP-3 complex subunit delta,Adaptor-related protein complex 3 subunit delta-1,Delta-adaptin |
| 2 | B, I (B, b) | AP-3 complex subunit beta-1 | polymer | 677 | 76499.6 | 2 | UniProt (O00203) Pfam (PF01602) | Homo sapiens (human) | Adaptor protein complex AP-3 subunit beta-1,Adaptor-related protein complex 3 subunit beta-1,Beta-3A-adaptin,Clathrin assembly protein complex 3 beta-1 large chain |
| 3 | C, J (M, m) | AP-3 complex subunit mu-1 | polymer | 418 | 46990.0 | 2 | UniProt (Q9Y2T2) Pfam (PF01217) Pfam (PF00928) | Homo sapiens (human) | AP-3 adaptor complex mu3A subunit,Adaptor-related protein complex 3 subunit mu-1,Mu-adaptin 3A,Mu3A-adaptin |
| 4 | D, K (S, s) | AP-3 complex subunit sigma-1 | polymer | 193 | 21755.1 | 2 | UniProt (Q92572) Pfam (PF01217) | Homo sapiens (human) | AP-3 complex subunit sigma-3A,Adaptor-related protein complex 3 subunit sigma-1,Clathrin-associated/assembly/adaptor protein,small 3,Sigma-3A-adaptin,Sigma3A-adaptin,Sigma-adaptin 3a |
| 5 | E, F, L, M (A, C, a, c) | ADP-ribosylation factor 1 | polymer | 182 | 20775.8 | 4 | UniProt (P84077) Pfam (PF00025) | Homo sapiens (human) | |
| 6 | G, N (Y, y) | Lysosome-associated membrane glycoprotein 1 | polymer | 12 | 1377.6 | 2 | UniProt (P11279) | Homo sapiens (human) | LAMP-1,Lysosome-associated membrane protein 1,CD107 antigen-like family member A |
| 7 | O, Q, S, U (A, C, a, c) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 8 | P, R, T, V (A, C, a, c) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 4 | Chemie (GTP) |
Sequence modifications
A, C, a, c: 2 - 181 (UniProt: P84077)
| PDB | External Database | Details |
|---|---|---|
| Leu 71 | Gln 71 | engineered mutation |
| Ser 182 | - | expression tag |
| Leu 183 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 515111.6 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 2189.9 | |
| All* | Total formula weight | 517301.5 |
