9BS4
DNA Ligase 1 E346A/E592A double mutant with 5'-rG:C
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E | DNA ligase 1 | polymer | 644 | 71581.9 | 2 | UniProt (P18858) Pfam (PF04675) Pfam (PF01068) Pfam (PF04679) In PDB | Homo sapiens (human) | DNA ligase I,Polydeoxyribonucleotide synthase [ATP] 1 |
| 2 | B, F | DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*TP*G)-3') | polymer | 11 | 3405.2 | 2 | synthetic construct | ||
| 3 | C, G | DNA/RNA (5'-R(P*G)-D(P*TP*CP*GP*GP*AP*C)-3') | polymer | 7 | 2154.4 | 2 | synthetic construct | ||
| 4 | D, H | DNA (5'-D(*GP*TP*CP*CP*GP*AP*CP*CP*AP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3') | polymer | 18 | 5446.5 | 2 | synthetic construct | ||
| 5 | A, E | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 2 | Chemie (AMP) | |||
| 6 | water | water | 18.0 | 377 | Chemie (HOH) |
Sequence modifications
A, E: 261 - 904 (UniProt: P18858)
| PDB | External Database | Details |
|---|---|---|
| Ala 346 | Glu 346 | engineered mutation |
| Ala 592 | Glu 592 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 165176.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 694.4 | |
| All* | Total formula weight | 165870.6 |
