9BKK

Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (mGsqi) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas	polymer	253	29145.0	1	UniProt (Q5JWF2) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein,Extra large alphas protein,XLalphas
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37413.9	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (P)	Cholecystokinin-8	polymer	9	1142.3	1	UniProt (P06307)	Homo sapiens	CCK-8
5	E (R)	Cholecystokinin receptor type A	polymer	427	47694.8	1	UniProt (P32238) Pfam (PF09193) Pfam (PF00001)	Homo sapiens (human)	CCK-A receptor,CCK-AR,Cholecystokinin-1 receptor,CCK1-R

Sequence modifications

A: 204 - 394 (UniProt: Q5JWF2)

PDB	External Database	Details
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Gly 9	-	expression tag
Cys 10	-	expression tag
Thr 11	-	expression tag
Leu 12	-	expression tag
Ser 13	-	expression tag
Ala 14	-	expression tag
Glu 15	-	expression tag
Asp 16	-	expression tag
Lys 17	-	expression tag
Ala 18	-	expression tag
Ala 19	-	expression tag
Val 20	-	expression tag
Glu 21	-	expression tag
Arg 22	-	expression tag
Ser 23	-	expression tag
Lys 24	-	expression tag
Met 25	-	expression tag
Ile 26	-	expression tag
Asp 27	-	expression tag
Arg 28	-	expression tag
Asn 29	-	expression tag
Leu 30	-	expression tag
Arg 31	-	expression tag
Glu 32	-	expression tag
Asp 33	-	expression tag
Gly 34	-	expression tag
Glu 35	-	expression tag
Lys 36	-	expression tag
Ala 37	-	expression tag
Arg 38	-	expression tag
Arg 39	-	expression tag
Thr 40	-	expression tag
Leu 41	-	expression tag
Arg 42	-	expression tag
Leu 43	-	expression tag
Leu 44	-	expression tag
Leu 45	-	expression tag
Leu 46	-	expression tag
Gly 47	-	expression tag
Ala 48	-	expression tag
Asp 49	-	expression tag
Asn 50	-	expression tag
Ser 51	-	expression tag
Gly 52	-	expression tag
Lys 53	-	expression tag
Ser 54	-	expression tag
Thr 55	-	expression tag
Ile 56	-	expression tag
Val 57	-	expression tag
Lys 58	-	expression tag
Gln 59	-	expression tag
Met 191	-	expression tag
Arg 192	-	expression tag
Ile 193	-	expression tag
Leu 194	-	expression tag
His 195	-	expression tag
Gly 196	-	expression tag
Gly 197	-	expression tag
Ser 198	-	expression tag
Gly 199	-	expression tag
Gly 200	-	expression tag
Ser 201	-	expression tag
Gly 202	-	expression tag
Gly 203	-	expression tag
Asp 249	Ala 892	engineered mutation
Asp 252	Ser 895	engineered mutation
-	Met 898	deletion
-	Val 899	deletion
-	Ile 900	deletion
-	Arg 901	deletion
-	Glu 902	deletion
-	Asp 903	deletion
-	Asn 904	deletion
-	Gln 905	deletion
-	Thr 906	deletion
-	Asn 907	deletion
Asp 272	Leu 915	engineered mutation
Lys 343	Asp 986	engineered mutation
Val 346	Leu 989	engineered mutation
Asp 347	Arg 990	engineered mutation
Ile 358	Tyr 1001	engineered mutation
Ala 372	Ile 1015	engineered mutation
Ile 375	Val 1018	engineered mutation
Lys 380	Arg 1023	engineered mutation
Leu 384	Gln 1027	engineered mutation
Gln 385	Arg 1028	engineered mutation
Asn 387	His 1030	engineered mutation
Glu 390	Gln 1033	engineered mutation
Asn 392	Glu 1035	engineered mutation
Val 394	Leu 1037	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Gln 1	-	expression tag

P: 1 - 8 (UniProt: P06307)

PDB	External Database	Details
Nh2 9	-	amidation

R: 2 - 428 (UniProt: P32238)

PDB	External Database	Details
Leu 130	Phe 130	engineered mutation
Ala 136	Ser 136	engineered mutation
Ser 141	Gly 141	engineered mutation
Leu 216	Ile 216	engineered mutation
Phe 219	Leu 219	engineered mutation
Val 223	Ile 223	engineered mutation
Ala 226	Met 226	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	123257.0
All*	Total formula weight	123257.0

*Water molecules are not included.