9BI4

cryo EM structure of dsDNA bound Mre11-Rad50 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (C, D)	DNA repair protein RAD50	polymer	1312	152797.7	2	UniProt (P12753) Pfam (PF13476) Pfam (PF04423) Pfam (PF13558)	Saccharomyces cerevisiae (brewer's yeast)	153 kDa protein
2	C, E (B, A)	Double-strand break repair protein MRE11	polymer	706	79607.3	2	UniProt (P32829) Pfam (PF00149) Pfam (PF04152)	Saccharomyces cerevisiae (brewer's yeast)
3	D (E)	one strand of dsDNA	polymer	83	25951.2	1		synthetic construct
4	F (F)	second strand of dsDNA	polymer	83	25203.0	1		synthetic construct
5	G, I (C, D)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
6	H, J (C, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	K, L, M, N (B, A)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)

Sequence modifications

C, D: 1 - 1312 (UniProt: P12753)

PDB	External Database	Details
Gln 1235	Glu 1235	engineered mutation

B, A: 1 - 692 (UniProt: P32829)

PDB	External Database	Details
Tyr 693	-	expression tag
Asp 694	-	expression tag
Tyr 695	-	expression tag
Lys 696	-	expression tag
Asp 697	-	expression tag
Asp 698	-	expression tag
Asp 699	-	expression tag
Asp 700	-	expression tag
Lys 701	-	expression tag
His 702	-	expression tag
His 703	-	expression tag
His 704	-	expression tag
His 705	-	expression tag
His 706	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	515964.2
Non-Polymers*	Number of molecules	8
	Total formula weight	1282.7
All*	Total formula weight	517246.9

*Water molecules are not included.