9B5F

Ubiquitin E1-Ub-E2 tetrahedral transthiolation intermediate mimic (doubly Ub-loaded) - cluster 1 map and model (Ub(A)/ATP/Mg)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin-activating enzyme E1 1	polymer	1001	111764.0	1	UniProt (O94609) Pfam (PF00899) Pfam (PF16190) Pfam (PF16191) Pfam (PF10585) Pfam (PF09358)	Schizosaccharomyces pombe 972h-	Poly(A)+ RNA transport protein 3
2	B (B)	Polyubiquitin	polymer	76	8568.8	1	UniProt (P0CG72) Pfam (PF00240)	Schizosaccharomyces pombe 972h-
3	C (C)	Ubiquitin-conjugating enzyme E2 4	polymer	153	17043.3	1	UniProt (P46595) Pfam (PF00179)	Schizosaccharomyces pombe 972h-	E2 ubiquitin-conjugating enzyme 4,Ubiquitin carrier protein 4,Ubiquitin-protein ligase 4
4	D (D)	Ubiquitin	polymer	79	8769.9	1	UniProt (P0CH07) Pfam (PF00240)	Schizosaccharomyces pombe 972h-
5	E, F (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	G (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
7	H (D)	4-aminobutanenitrile	non-polymer		84.1	1	Chemie (A1AIV)

Sequence modifications

A: 13 - 1012 (UniProt: O94609)

PDB	External Database	Details
Ser 12	-	expression tag

C: 1 - 147 (UniProt: P46595)

PDB	External Database	Details
Ser 21	Cys 21	engineered mutation
Ser 107	Cys 107	engineered mutation
Gly 148	-	expression tag
Gly 149	-	expression tag
Leu 150	-	expression tag
Val 151	-	expression tag
Pro 152	-	expression tag
Arg 153	-	expression tag

D: 79 - 153 (UniProt: P0CH07)

PDB	External Database	Details
Gly 75	-	expression tag
Ser 76	-	expression tag
Gly 77	-	expression tag
Gly 78	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	146146.1
Non-Polymers*	Number of molecules	4
	Total formula weight	639.9
All*	Total formula weight	146786.0

*Water molecules are not included.