9B59
Ubiquitin E2-Ub-E3 HECT tetrahedral transthiolation intermediate mimic - state 5
This is a non-PDB format compatible entry.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Ubiquitin-conjugating enzyme E2 4 | polymer | 153 | 17043.3 | 1 | UniProt (P46595) Pfam (PF00179) In PDB | Schizosaccharomyces pombe 972h- | E2 ubiquitin-conjugating enzyme 4,Ubiquitin carrier protein 4,Ubiquitin-protein ligase 4 |
2 | B | Ubiquitin | polymer | 79 | 8769.9 | 1 | UniProt (P0CH07) Pfam (PF00240) In PDB | Schizosaccharomyces pombe 972h- | |
3 | C | E3 ubiquitin-protein ligase pub2 | polymer | 380 | 44231.8 | 1 | UniProt (Q9UTG2) Pfam (PF00632) In PDB | Schizosaccharomyces pombe 972h- | HECT-type E3 ubiquitin transferase pub2 |
4 | B | 4-aminobutanenitrile | non-polymer | 84.1 | 1 | Chemie (A1AIV) |
Sequence modifications
A: 1 - 147 (UniProt: P46595)
B: 1 - 75 (UniProt: P0CH07)
C: 295 - 671 (UniProt: Q9UTG2)
PDB | External Database | Details |
---|---|---|
Ser 21 | Cys 21 | engineered mutation |
Ser 107 | Cys 107 | engineered mutation |
Gly 148 | - | expression tag |
Gly 149 | - | expression tag |
Leu 150 | - | expression tag |
Val 151 | - | expression tag |
Pro 152 | - | expression tag |
Arg 153 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Gly -1 | - | expression tag |
Gly 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ser 292 | - | expression tag |
His 293 | - | expression tag |
Met 294 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 70045.1 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 84.1 | |
All* | Total formula weight | 70129.2 |