9B59
Ubiquitin E2-Ub-E3 HECT tetrahedral transthiolation intermediate mimic - state 5
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.49 |
Software | Topaz,cryoSPARC,PHENIX,SerialEM,Gctf,RELION |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |