9B55

Ubiquitin E2-Ub-E3 HECT tetrahedral transthiolation intermediate mimic - state 1

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitin-conjugating enzyme E2 4	polymer	153	17043.3	1	UniProt (P46595) Pfam (PF00179) In PDB	Schizosaccharomyces pombe 972h-	E2 ubiquitin-conjugating enzyme 4,Ubiquitin carrier protein 4,Ubiquitin-protein ligase 4
2	B	Ubiquitin	polymer	79	8769.9	1	UniProt (P0CH07) Pfam (PF00240) In PDB	Schizosaccharomyces pombe 972h-
3	C	E3 ubiquitin-protein ligase pub2	polymer	380	44231.8	1	UniProt (Q9UTG2) Pfam (PF00632) In PDB	Schizosaccharomyces pombe 972h-	HECT-type E3 ubiquitin transferase pub2
4	B	4-aminobutanenitrile	non-polymer		84.1	1	Chemie (A1AIV)

Sequence modifications

A: 1 - 147 (UniProt: P46595)

PDB	External Database	Details
Ser 21	Cys 21	engineered mutation
Ser 107	Cys 107	engineered mutation
Gly 148	-	expression tag
Gly 149	-	expression tag
Leu 150	-	expression tag
Val 151	-	expression tag
Pro 152	-	expression tag
Arg 153	-	expression tag

B: 1 - 75 (UniProt: P0CH07)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag

C: 295 - 671 (UniProt: Q9UTG2)

PDB	External Database	Details
Ser 292	-	expression tag
His 293	-	expression tag
Met 294	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	70045.1
Non-Polymers*	Number of molecules	1
	Total formula weight	84.1
All*	Total formula weight	70129.2

*Water molecules are not included.