9B3I
Cryo-EM structure of yeast (Nap1)2-H2A-H2B-Kap114-RanGTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | KAP114 isoform 1 | polymer | 1012 | 114833.5 | 1 | UniProt (A0A8H4BZV8) Pfam (PF03810) Pfam (PF25018) UniProt (by SIFTS) (P53067) | Saccharomyces cerevisiae (brewer's yeast) | |
| 2 | B (B) | Histone H2A | polymer | 131 | 13882.0 | 1 | UniProt (A0A6A5Q402) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P04912) | Saccharomyces cerevisiae (brewer's yeast) | |
| 3 | C (C) | Histone H2B | polymer | 130 | 14133.1 | 1 | UniProt (A0A6A5Q1U6) Pfam (PF00125) UniProt (by SIFTS) (P02294) | Saccharomyces cerevisiae (brewer's yeast) | |
| 4 | D (D) | GTP-binding nuclear protein | polymer | 186 | 21283.5 | 1 | UniProt (A0A6A5PUD0) Pfam (PF00071) UniProt (by SIFTS) (P32835) | Saccharomyces cerevisiae (brewer's yeast) | |
| 5 | E, F (E, F) | NAP1 isoform 1 | polymer | 420 | 48035.4 | 2 | UniProt (A0A8H4BY55) Pfam (PF00956) UniProt (by SIFTS) (P25293) | Saccharomyces cerevisiae (brewer's yeast) | |
| 6 | G (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 7 | H (D) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) |
Sequence modifications
A: 1 - 1004 (UniProt: A0A8H4BZV8)
D: 2 - 179 (UniProt: A0A6A5PUD0)
E, F: 3 - 417 (UniProt: A0A8H4BY55)
| PDB | External Database | Details |
|---|---|---|
| Gly -7 | - | expression tag |
| Ser -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Asn -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Arg -2 | - | expression tag |
| Val -1 | - | expression tag |
| Asp 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Ala 1 | - | expression tag |
| Leu 71 | Gln 71 | engineered mutation |
| Glu 180 | - | expression tag |
| Asn 181 | - | expression tag |
| Leu 182 | - | expression tag |
| Tyr 183 | - | expression tag |
| Phe 184 | - | expression tag |
| Gln 185 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Met 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Thr 2 | - | expression tag |
| Ala 200 | Cys 200 | engineered mutation |
| Ala 249 | Cys 249 | engineered mutation |
| Ala 272 | Cys 272 | engineered mutation |
| Asp 384 | Glu 384 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 260202.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 547.5 | |
| All* | Total formula weight | 260750.4 |
