9B3I
Cryo-EM structure of yeast (Nap1)2-H2A-H2B-Kap114-RanGTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | KAP114 isoform 1 | polymer | 1012 | 114833.5 | 1 | UniProt (A0A8H4BZV8) Pfam (PF03810) Pfam (PF25018) UniProt (by SIFTS) (P53067) | Saccharomyces cerevisiae (brewer's yeast) | |
| 2 | B (B) | Histone H2A | polymer | 131 | 13882.0 | 1 | UniProt (A0A6A5Q402) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P04912) | Saccharomyces cerevisiae (brewer's yeast) | |
| 3 | C (C) | Histone H2B | polymer | 130 | 14133.1 | 1 | UniProt (A0A6A5Q1U6) Pfam (PF00125) UniProt (by SIFTS) (P02294) | Saccharomyces cerevisiae (brewer's yeast) | |
| 4 | D (D) | GTP-binding nuclear protein | polymer | 186 | 21283.5 | 1 | UniProt (A0A6A5PUD0) Pfam (PF00071) UniProt (by SIFTS) (P32835) | Saccharomyces cerevisiae (brewer's yeast) | |
| 5 | E, F (E, F) | NAP1 isoform 1 | polymer | 420 | 48035.4 | 2 | UniProt (A0A8H4BY55) Pfam (PF00956) UniProt (by SIFTS) (P25293) | Saccharomyces cerevisiae (brewer's yeast) | |
| 6 | G (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 7 | H (D) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) |
Sequence modifications
A: 1 - 1004 (UniProt: A0A8H4BZV8)
D: 2 - 179 (UniProt: A0A6A5PUD0)
E, F: 3 - 417 (UniProt: A0A8H4BY55)
| PDB | External Database | Details |
|---|---|---|
| Gly -7 | - | expression tag |
| Ser -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Asn -4 | - | expression tag |
| Ser -3 | - | expression tag |
| Arg -2 | - | expression tag |
| Val -1 | - | expression tag |
| Asp 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Ala 1 | - | expression tag |
| Leu 71 | Gln 71 | engineered mutation |
| Glu 180 | - | expression tag |
| Asn 181 | - | expression tag |
| Leu 182 | - | expression tag |
| Tyr 183 | - | expression tag |
| Phe 184 | - | expression tag |
| Gln 185 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Met 0 | - | expression tag |
| Gly 1 | - | expression tag |
| Thr 2 | - | expression tag |
| Ala 200 | Cys 200 | engineered mutation |
| Ala 249 | Cys 249 | engineered mutation |
| Ala 272 | Cys 272 | engineered mutation |
| Asp 384 | Glu 384 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 260202.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 547.5 | |
| All* | Total formula weight | 260750.4 |
