9B3I
Cryo-EM structure of yeast (Nap1)2-H2A-H2B-Kap114-RanGTP
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.88 |
| Number of Particle | 1381753 |
| Software | cryoSPARC,PHENIX,SerialEM,UCSF ChimeraX,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
