9B0T
Cryo-EM structure of E227Q variant of uMtCK1 in complex with transition state analog
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Creatine kinase U-type, mitochondrial | polymer | 418 | 47597.7 | 8 | UniProt (P12532) Pfam (PF02807) Pfam (PF00217) | Homo sapiens (human) | Acidic-type mitochondrial creatine kinase,Mia-CK,Ubiquitous mitochondrial creatine kinase,U-MtCK |
| 2 | CA, GA, I, KA, M... (F, G, A, H, B...) | N-[(E)-AMINO(IMINO)METHYL]-N-METHYLGLYCINE | non-polymer | 131.1 | 8 | Chemie (CRN) | |||
| 3 | DA, HA, J, LA, N... (F, G, A, H, B...) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 8 | Chemie (ADP) | |||
| 4 | AA, EA, IA, K, MA... (E, F, G, A, H...) | NITRATE ION | non-polymer | 62.0 | 8 | Chemie (NO3) | |||
| 5 | BA, FA, JA, L, NA... (E, F, G, A, H...) | MAGNESIUM ION | non-polymer | 24.3 | 8 | Chemie (MG) |
Sequence modifications
A, B, C, D, E, F, G, H: 1 - 379 (UniProt: P12532)
| PDB | External Database | Details |
|---|---|---|
| Met -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Ser -25 | - | expression tag |
| Ser -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| Gly -15 | - | expression tag |
| Leu -14 | - | expression tag |
| Val -13 | - | expression tag |
| Pro -12 | - | expression tag |
| Arg -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Ser -9 | - | expression tag |
| His -8 | - | expression tag |
| Met -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Asn -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Gln 227 | Glu 265 | engineered mutation |
| Gly 380 | - | expression tag |
| Ser 381 | - | expression tag |
| Ser 382 | - | expression tag |
| Asp 383 | - | expression tag |
| Tyr 384 | - | expression tag |
| Lys 385 | - | expression tag |
| Asp 386 | - | expression tag |
| Asp 387 | - | expression tag |
| Asp 388 | - | expression tag |
| Asp 389 | - | expression tag |
| Lys 390 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 380781.9 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 5157.2 | |
| All* | Total formula weight | 385939.0 |
