9AZJ

Structure of ubiquitinated NEMO UBAN K285C-Ub G76C bound to HOIP NZF1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	NF-kappa-B essential modulator	polymer	108	12643.4	4	UniProt (Q9Y6K9) Pfam (PF16516)	Homo sapiens (human)	NEMO,FIP-3,IkB kinase-associated protein 1,IKKAP1,Inhibitor of nuclear factor kappa-B kinase subunit gamma,I-kappa-B kinase subunit gamma,IKK-gamma,IKKG,IkB kinase subunit gamma,NF-kappa-B essential modifier
2	E, G (E, G)	E3 ubiquitin-protein ligase RNF31	polymer	29	3245.7	2	UniProt (Q96EP0)	Homo sapiens (human)	HOIL-1-interacting protein,HOIP,RING finger protein 31,RING-type E3 ubiquitin transferase RNF31,Zinc in-between-RING-finger ubiquitin-associated domain protein
3	F, H, I (F, S, Z)	Ubiquitin	polymer	76	8622.9	3	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (human)
4	J, K (E, G)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A, B, C, D: 257 - 364 (UniProt: Q9Y6K9)

PDB	External Database	Details
Cys 285	Lys 285	engineered mutation

F, S, Z: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Cys 76	Gly 76	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	82933.8
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	83064.6

*Water molecules are not included.