9AYF
Structure of human calcium-sensing receptor in complex with Gi1 (miniGi1) protein in detergent
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (Q, R) | Isoform 1 of Extracellular calcium-sensing receptor | polymer | 911 | 102864.6 | 2 | UniProt (P41180) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003) | Homo sapiens (human) | CaR,CaSR,hCasR,Parathyroid cell calcium-sensing receptor 1,PCaR1 |
| 2 | C (H) | Single-chain antibody fragment scFv16 | polymer | 297 | 31870.6 | 1 | Mus musculus | ||
| 3 | D (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 229 | 25985.7 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 4 | E (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 348 | 38413.9 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 5 | F (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 6 | G, H, I, J (C, D, E, F) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
| 7 | K, L, M, U, V... (Q, R) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 6 | Chemie (NAG) | |||
| 8 | N, X (Q, R) | CYCLOMETHYLTRYPTOPHAN | non-polymer | 216.2 | 2 | Chemie (TCR) | |||
| 9 | O, Y (Q, R) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
| 10 | AA, BA, CA, P, Q... (R, Q) | CALCIUM ION | non-polymer | 40.1 | 8 | Chemie (CA) | |||
| 11 | EA, T (R, Q) | 3-(2-chlorophenyl)-N-[(1R)-1-(3-methoxyphenyl)ethyl]propan-1-amine | non-polymer | 303.8 | 2 | Chemie (9IG) | |||
| 12 | DA (R) | Lauryl Maltose Neopentyl Glycol | non-polymer | 1005.2 | 1 | Chemie (AV0) |
Sequence modifications
Q, R: -7 - 903 (UniProt: P41180)
A: 1 - 57 (UniProt: P63096)
A: 66 - 229 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asp 12 | - | insertion |
| Tyr 13 | - | insertion |
| Lys 14 | - | insertion |
| Asp 15 | - | insertion |
| Asp 16 | - | insertion |
| Asp 17 | - | insertion |
| Asp 18 | - | insertion |
| Lys 19 | - | insertion |
| PDB | External Database | Details |
|---|---|---|
| Asp 42 | Gly 42 | engineered mutation |
| Asn 43 | Glu 43 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 58 | - | linker |
| Gly 59 | - | linker |
| Gly 60 | - | linker |
| Gly 61 | - | linker |
| Gly 62 | - | linker |
| Gly 63 | - | linker |
| Gly 64 | - | linker |
| Gly 65 | - | linker |
| Asp 102 | Gly 217 | engineered mutation |
| Ala 104 | Thr 219 | variant |
| Asp 111 | Ala 226 | engineered mutation |
| - | Asp 231 | deletion |
| - | Leu 232 | deletion |
| - | Val 233 | deletion |
| - | Leu 234 | deletion |
| - | Ala 235 | deletion |
| - | Glu 236 | deletion |
| - | Asp 237 | deletion |
| - | Glu 238 | deletion |
| - | Glu 239 | deletion |
| - | Met 240 | deletion |
| Gln 163 | Pro 288 | variant |
| Ala 207 | Val 332 | engineered mutation |
| Ile 210 | Val 335 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -7 | - | initiating methionine |
| Asp -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Lys 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 309860.6 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 1697.6 | |
| Non-Polymers* | Number of molecules | 21 |
| Total formula weight | 3883.1 | |
| All* | Total formula weight | 315441.3 |
