9AYF
Structure of human calcium-sensing receptor in complex with Gi1 (miniGi1) protein in detergent
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | Q, R | Isoform 1 of Extracellular calcium-sensing receptor | polymer | 911 | 102864.6 | 2 | UniProt (P41180) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003) In PDB | Homo sapiens (human) | CaR,CaSR,hCasR,Parathyroid cell calcium-sensing receptor 1,PCaR1 |
2 | H | Single-chain antibody fragment scFv16 | polymer | 297 | 31870.6 | 1 | Mus musculus | ||
3 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 229 | 25985.7 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
4 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 348 | 38413.9 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
5 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) In PDB | Homo sapiens (human) | G gamma-I |
6 | C, D, E, F | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
7 | Q, R | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 6 | Chemie (NAG) | |||
8 | Q, R | CYCLOMETHYLTRYPTOPHAN | non-polymer | 216.2 | 2 | Chemie (TCR) | |||
9 | Q, R | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
10 | R, Q | CALCIUM ION | non-polymer | 40.1 | 8 | Chemie (CA) | |||
11 | R, Q | 3-(2-chlorophenyl)-N-[(1R)-1-(3-methoxyphenyl)ethyl]propan-1-amine | non-polymer | 303.8 | 2 | Chemie (9IG) | |||
12 | R | Lauryl Maltose Neopentyl Glycol | non-polymer | 1005.2 | 1 | Chemie (AV0) |
Sequence modifications
Q, R: -7 - 903 (UniProt: P41180)
A: 1 - 57 (UniProt: P63096)
A: 66 - 229 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
PDB | External Database | Details |
---|---|---|
Asp 12 | - | insertion |
Tyr 13 | - | insertion |
Lys 14 | - | insertion |
Asp 15 | - | insertion |
Asp 16 | - | insertion |
Asp 17 | - | insertion |
Asp 18 | - | insertion |
Lys 19 | - | insertion |
PDB | External Database | Details |
---|---|---|
Asp 42 | Gly 42 | engineered mutation |
Asn 43 | Glu 43 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 58 | - | linker |
Gly 59 | - | linker |
Gly 60 | - | linker |
Gly 61 | - | linker |
Gly 62 | - | linker |
Gly 63 | - | linker |
Gly 64 | - | linker |
Gly 65 | - | linker |
Asp 102 | Gly 217 | engineered mutation |
Ala 104 | Thr 219 | variant |
Asp 111 | Ala 226 | engineered mutation |
- | Asp 231 | deletion |
- | Leu 232 | deletion |
- | Val 233 | deletion |
- | Leu 234 | deletion |
- | Ala 235 | deletion |
- | Glu 236 | deletion |
- | Asp 237 | deletion |
- | Glu 238 | deletion |
- | Glu 239 | deletion |
- | Met 240 | deletion |
Gln 163 | Pro 288 | variant |
Ala 207 | Val 332 | engineered mutation |
Ile 210 | Val 335 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -7 | - | initiating methionine |
Asp -6 | - | expression tag |
Tyr -5 | - | expression tag |
Lys -4 | - | expression tag |
Asp -3 | - | expression tag |
Asp -2 | - | expression tag |
Asp -1 | - | expression tag |
Asp 0 | - | expression tag |
Lys 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 309860.6 | |
Branched | Number of molecules | 4 |
Total formula weight | 1697.6 | |
Non-Polymers* | Number of molecules | 21 |
Total formula weight | 3883.1 | |
All* | Total formula weight | 315441.3 |