9AXC
Activated CRAF/MEK heterotetramer from focused refinement of CRAF/MEK/14-3-3 complex
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C | GST26/CRAF chimera | polymer | 563 | 64718.5 | 2 | UniProt (P08515) UniProt (P04049) Pfam (PF02798) Pfam (PF14497) Pfam (PF00069) In PDB | Homo sapiens (human) | Proto-oncogene c-RAF,cRaf,Raf-1 |
| 2 | B, D | Dual specificity mitogen-activated protein kinase kinase 1 | polymer | 394 | 43519.0 | 2 | UniProt (Q02750) Pfam (PF00069) In PDB | Homo sapiens (human) | MAP kinase kinase 1,MAPKK 1,MKK1,ERK activator kinase 1,MAPK/ERK kinase 1,MEK 1 |
| 3 | B, D | N-[3-fluoro-4-({7-[(3-fluoropyridin-2-yl)oxy]-4-methyl-2-oxo-2H-1-benzopyran-3-yl}methyl)pyridin-2-yl]-N'-methylsulfuric diamide | non-polymer | 488.5 | 2 | Chemie (A1AHE) |
Sequence modifications
A, C: 86 - 303 (UniProt: P08515)
A, C: 306 - 648 (UniProt: P04049)
B, D: 1 - 393 (UniProt: Q02750)
| PDB | External Database | Details |
|---|---|---|
| Ser 304 | - | linker |
| Asp 305 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Asp 340 | Tyr 340 | engineered mutation |
| Asp 341 | Tyr 341 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Ala 218 | Ser 218 | engineered mutation |
| Ala 222 | Ser 222 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 216475.0 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 976.9 | |
| All* | Total formula weight | 217451.9 |
