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9AXC

Activated CRAF/MEK heterotetramer from focused refinement of CRAF/MEK/14-3-3 complex

This is a non-PDB format compatible entry.
Functional Information from GO Data
ChainGOidnamespacecontents
A0004364molecular_functionglutathione transferase activity
A0004672molecular_functionprotein kinase activity
A0005524molecular_functionATP binding
A0006468biological_processprotein phosphorylation
A0006749biological_processglutathione metabolic process
A0016740molecular_functiontransferase activity
B0000165biological_processMAPK cascade
B0000166molecular_functionnucleotide binding
B0000187biological_processobsolete activation of MAPK activity
B0003056biological_processregulation of vascular associated smooth muscle contraction
B0004672molecular_functionprotein kinase activity
B0004674molecular_functionprotein serine/threonine kinase activity
B0004708molecular_functionMAP kinase kinase activity
B0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
B0004713molecular_functionprotein tyrosine kinase activity
B0005078molecular_functionMAP-kinase scaffold activity
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005769cellular_componentearly endosome
B0005770cellular_componentlate endosome
B0005783cellular_componentendoplasmic reticulum
B0005794cellular_componentGolgi apparatus
B0005813cellular_componentcentrosome
B0005829cellular_componentcytosol
B0005856cellular_componentcytoskeleton
B0005874cellular_componentmicrotubule
B0005886cellular_componentplasma membrane
B0005925cellular_componentfocal adhesion
B0005938cellular_componentcell cortex
B0006468biological_processprotein phosphorylation
B0006935biological_processchemotaxis
B0006979biological_processresponse to oxidative stress
B0007165biological_processsignal transduction
B0007507biological_processheart development
B0008285biological_processnegative regulation of cell population proliferation
B0010508biological_processpositive regulation of autophagy
B0010628biological_processpositive regulation of gene expression
B0010629biological_processnegative regulation of gene expression
B0014044biological_processSchwann cell development
B0014069cellular_componentpostsynaptic density
B0016020cellular_componentmembrane
B0016301molecular_functionkinase activity
B0016740molecular_functiontransferase activity
B0019901molecular_functionprotein kinase binding
B0021697biological_processcerebellar cortex formation
B0021953biological_processcentral nervous system neuron differentiation
B0030182biological_processneuron differentiation
B0030216biological_processkeratinocyte differentiation
B0030295molecular_functionprotein kinase activator activity
B0030335biological_processpositive regulation of cell migration
B0030424cellular_componentaxon
B0030425cellular_componentdendrite
B0030878biological_processthyroid gland development
B0031267molecular_functionsmall GTPase binding
B0031435molecular_functionmitogen-activated protein kinase kinase kinase binding
B0032402biological_processmelanosome transport
B0032839cellular_componentdendrite cytoplasm
B0032872biological_processregulation of stress-activated MAPK cascade
B0032968biological_processpositive regulation of transcription elongation by RNA polymerase II
B0034111biological_processnegative regulation of homotypic cell-cell adhesion
B0035987biological_processendodermal cell differentiation
B0036064cellular_componentciliary basal body
B0038133biological_processERBB2-ERBB3 signaling pathway
B0042552biological_processmyelination
B0043204cellular_componentperikaryon
B0043410biological_processpositive regulation of MAPK cascade
B0043539molecular_functionprotein serine/threonine kinase activator activity
B0044342biological_processtype B pancreatic cell proliferation
B0044877molecular_functionprotein-containing complex binding
B0045893biological_processpositive regulation of DNA-templated transcription
B0045933biological_processpositive regulation of muscle contraction
B0046579biological_processpositive regulation of Ras protein signal transduction
B0047496biological_processvesicle transport along microtubule
B0048009biological_processinsulin-like growth factor receptor signaling pathway
B0048313biological_processGolgi inheritance
B0048471cellular_componentperinuclear region of cytoplasm
B0048538biological_processthymus development
B0048678biological_processresponse to axon injury
B0048679biological_processregulation of axon regeneration
B0048812biological_processneuron projection morphogenesis
B0048870biological_processcell motility
B0050772biological_processpositive regulation of axonogenesis
B0051384biological_processresponse to glucocorticoid
B0060020biological_processBergmann glial cell differentiation
B0060324biological_processface development
B0060425biological_processlung morphogenesis
B0060440biological_processtrachea formation
B0060502biological_processepithelial cell proliferation involved in lung morphogenesis
B0060674biological_processplacenta blood vessel development
B0060711biological_processlabyrinthine layer development
B0070328biological_processtriglyceride homeostasis
B0070371biological_processERK1 and ERK2 cascade
B0070372biological_processregulation of ERK1 and ERK2 cascade
B0070374biological_processpositive regulation of ERK1 and ERK2 cascade
B0071902biological_processpositive regulation of protein serine/threonine kinase activity
B0090170biological_processregulation of Golgi inheritance
B0090398biological_processcellular senescence
B0097110molecular_functionscaffold protein binding
B0098696biological_processregulation of neurotransmitter receptor localization to postsynaptic specialization membrane
B0098978cellular_componentglutamatergic synapse
B0106310molecular_functionprotein serine kinase activity
B1903226biological_processpositive regulation of endodermal cell differentiation
B1903298biological_processnegative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
B2000641biological_processregulation of early endosome to late endosome transport
B2001171biological_processpositive regulation of ATP biosynthetic process
C0004364molecular_functionglutathione transferase activity
C0004672molecular_functionprotein kinase activity
C0005524molecular_functionATP binding
C0006468biological_processprotein phosphorylation
C0006749biological_processglutathione metabolic process
C0016740molecular_functiontransferase activity
D0000165biological_processMAPK cascade
D0000166molecular_functionnucleotide binding
D0000187biological_processobsolete activation of MAPK activity
D0003056biological_processregulation of vascular associated smooth muscle contraction
D0004672molecular_functionprotein kinase activity
D0004674molecular_functionprotein serine/threonine kinase activity
D0004708molecular_functionMAP kinase kinase activity
D0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
D0004713molecular_functionprotein tyrosine kinase activity
D0005078molecular_functionMAP-kinase scaffold activity
D0005515molecular_functionprotein binding
D0005524molecular_functionATP binding
D0005634cellular_componentnucleus
D0005737cellular_componentcytoplasm
D0005739cellular_componentmitochondrion
D0005769cellular_componentearly endosome
D0005770cellular_componentlate endosome
D0005783cellular_componentendoplasmic reticulum
D0005794cellular_componentGolgi apparatus
D0005813cellular_componentcentrosome
D0005829cellular_componentcytosol
D0005856cellular_componentcytoskeleton
D0005874cellular_componentmicrotubule
D0005886cellular_componentplasma membrane
D0005925cellular_componentfocal adhesion
D0005938cellular_componentcell cortex
D0006468biological_processprotein phosphorylation
D0006935biological_processchemotaxis
D0006979biological_processresponse to oxidative stress
D0007165biological_processsignal transduction
D0007507biological_processheart development
D0008285biological_processnegative regulation of cell population proliferation
D0010508biological_processpositive regulation of autophagy
D0010628biological_processpositive regulation of gene expression
D0010629biological_processnegative regulation of gene expression
D0014044biological_processSchwann cell development
D0014069cellular_componentpostsynaptic density
D0016020cellular_componentmembrane
D0016301molecular_functionkinase activity
D0016740molecular_functiontransferase activity
D0019901molecular_functionprotein kinase binding
D0021697biological_processcerebellar cortex formation
D0021953biological_processcentral nervous system neuron differentiation
D0030182biological_processneuron differentiation
D0030216biological_processkeratinocyte differentiation
D0030295molecular_functionprotein kinase activator activity
D0030335biological_processpositive regulation of cell migration
D0030424cellular_componentaxon
D0030425cellular_componentdendrite
D0030878biological_processthyroid gland development
D0031267molecular_functionsmall GTPase binding
D0031435molecular_functionmitogen-activated protein kinase kinase kinase binding
D0032402biological_processmelanosome transport
D0032839cellular_componentdendrite cytoplasm
D0032872biological_processregulation of stress-activated MAPK cascade
D0032968biological_processpositive regulation of transcription elongation by RNA polymerase II
D0034111biological_processnegative regulation of homotypic cell-cell adhesion
D0035987biological_processendodermal cell differentiation
D0036064cellular_componentciliary basal body
D0038133biological_processERBB2-ERBB3 signaling pathway
D0042552biological_processmyelination
D0043204cellular_componentperikaryon
D0043410biological_processpositive regulation of MAPK cascade
D0043539molecular_functionprotein serine/threonine kinase activator activity
D0044342biological_processtype B pancreatic cell proliferation
D0044877molecular_functionprotein-containing complex binding
D0045893biological_processpositive regulation of DNA-templated transcription
D0045933biological_processpositive regulation of muscle contraction
D0046579biological_processpositive regulation of Ras protein signal transduction
D0047496biological_processvesicle transport along microtubule
D0048009biological_processinsulin-like growth factor receptor signaling pathway
D0048313biological_processGolgi inheritance
D0048471cellular_componentperinuclear region of cytoplasm
D0048538biological_processthymus development
D0048678biological_processresponse to axon injury
D0048679biological_processregulation of axon regeneration
D0048812biological_processneuron projection morphogenesis
D0048870biological_processcell motility
D0050772biological_processpositive regulation of axonogenesis
D0051384biological_processresponse to glucocorticoid
D0060020biological_processBergmann glial cell differentiation
D0060324biological_processface development
D0060425biological_processlung morphogenesis
D0060440biological_processtrachea formation
D0060502biological_processepithelial cell proliferation involved in lung morphogenesis
D0060674biological_processplacenta blood vessel development
D0060711biological_processlabyrinthine layer development
D0070328biological_processtriglyceride homeostasis
D0070371biological_processERK1 and ERK2 cascade
D0070372biological_processregulation of ERK1 and ERK2 cascade
D0070374biological_processpositive regulation of ERK1 and ERK2 cascade
D0071902biological_processpositive regulation of protein serine/threonine kinase activity
D0090170biological_processregulation of Golgi inheritance
D0090398biological_processcellular senescence
D0097110molecular_functionscaffold protein binding
D0098696biological_processregulation of neurotransmitter receptor localization to postsynaptic specialization membrane
D0098978cellular_componentglutamatergic synapse
D0106310molecular_functionprotein serine kinase activity
D1903226biological_processpositive regulation of endodermal cell differentiation
D1903298biological_processnegative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
D2000641biological_processregulation of early endosome to late endosome transport
D2001171biological_processpositive regulation of ATP biosynthetic process
Functional Information from PROSITE/UniProt
site_idPS00107
Number of Residues24
DetailsPROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGAGNGGVVFkVshkpsglv..........MARK
ChainResidueDetails
BLEU74-LYS97
AILE355-LYS375

site_idPS00108
Number of Residues13
DetailsPROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. ImHrDVKpsNILV
ChainResidueDetails
BILE186-VAL198
AILE464-LEU476

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues2
DetailsACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10027
ChainResidueDetails
BASP190
DASP190
AASN139
AGLN152
CTYR92
CTRP126
CASN139
CGLN152

site_idSWS_FT_FI2
Number of Residues10
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000269|PubMed:15543157, ECO:0000269|PubMed:17880056, ECO:0000269|PubMed:18951019, ECO:0000269|PubMed:19019675, ECO:0000269|PubMed:19706763, ECO:0000269|PubMed:21310613
ChainResidueDetails
BLEU74
DASP208
BMET143
BSER150
BLYS192
BASP208
DLEU74
DMET143
DSER150
DLYS192

site_idSWS_FT_FI3
Number of Residues2
DetailsBINDING: BINDING => ECO:0000269|PubMed:19161339, ECO:0007744|PDB:3EQH
ChainResidueDetails
BLYS97
DLYS97

site_idSWS_FT_FI4
Number of Residues4
DetailsBINDING: BINDING => ECO:0000269|PubMed:19161339, ECO:0007744|PDB:3EQF
ChainResidueDetails
BGLU144
BSER194
DGLU144
DSER194

site_idSWS_FT_FI5
Number of Residues2
DetailsSITE: Cleavage; by anthrax lethal factor
ChainResidueDetails
BPRO8
DPRO8

site_idSWS_FT_FI6
Number of Residues2
DetailsMOD_RES: Phosphoserine; by BRAF and RAF1 => ECO:0000269|PubMed:10409742, ECO:0000269|PubMed:20956560, ECO:0000269|PubMed:29433126, ECO:0000269|PubMed:8131746
ChainResidueDetails
BALA218
DALA218

site_idSWS_FT_FI7
Number of Residues2
DetailsMOD_RES: Phosphoserine; by BRAF and RAF1 => ECO:0000269|PubMed:20956560, ECO:0000269|PubMed:29433126, ECO:0000269|PubMed:8131746
ChainResidueDetails
BALA222
DALA222
CASP340
CASP341

site_idSWS_FT_FI8
Number of Residues2
DetailsMOD_RES: Phosphothreonine => ECO:0007744|PubMed:18691976
ChainResidueDetails
BTHR286
DTHR286

site_idSWS_FT_FI9
Number of Residues2
DetailsMOD_RES: Phosphothreonine; by MAPK1 => ECO:0000250|UniProtKB:Q01986
ChainResidueDetails
BTHR292
DTHR292

site_idSWS_FT_FI10
Number of Residues2
DetailsMOD_RES: Phosphoserine; by PAK => ECO:0000269|PubMed:16129686
ChainResidueDetails
BSER298
DSER298

site_idSWS_FT_FI11
Number of Residues2
DetailsMOD_RES: Phosphoserine; by PKC => ECO:0000269|PubMed:8349614
ChainResidueDetails
ASER499
CSER499

site_idSWS_FT_FI12
Number of Residues2
DetailsMOD_RES: Symmetric dimethylarginine; by PRMT5 => ECO:0000269|PubMed:21917714
ChainResidueDetails
AARG563
CARG563

site_idSWS_FT_FI13
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0000269|PubMed:10801873, ECO:0000269|PubMed:16892053, ECO:0000269|PubMed:21917714, ECO:0000269|PubMed:8349614
ChainResidueDetails
ASER621
CSER621

site_idSWS_FT_FI14
Number of Residues2
DetailsMOD_RES: Phosphoserine; by MAPK1 => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163
ChainResidueDetails
ASER642
CSER642

237735

PDB entries from 2025-06-18

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