9AUC

Human Amylin1 Receptor in Complex with Gs and human Calcitonin Gene-Related Peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45683.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	138	15140.7	1		Lama glama
5	E (E)	Receptor activity-modifying protein 1	polymer	149	17066.7	1	UniProt (O60894) Pfam (PF04901)	Homo sapiens (human)	Calcitonin-receptor-like receptor activity-modifying protein 1,CRLR activity-modifying protein 1
6	F (P)	Calcitonin gene-related peptide 1	polymer	37	3796.4	1	UniProt (P06881)	Homo sapiens	Alpha-type CGRP,Calcitonin gene-related peptide I,CGRP-I
7	G (R)	Calcitonin receptor	polymer	501	58469.6	1	UniProt (P30988) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (human)	CT-R
8	H, I, M, N, O (P, R)	PALMITIC ACID	non-polymer		256.4	5	Chemie (PLM)
9	J, K, L (R)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
10	P, Q (R)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	2	Chemie (Y01)
11	R, S (P, R)	water	water		18.0	16	Chemie (HOH)

Sequence modifications

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

E: 27 - 148 (UniProt: O60894)

PDB	External Database	Details
Met 0	-	expression tag
Lys 1	-	expression tag
Thr 2	-	expression tag
Ile 3	-	expression tag
Ile 4	-	expression tag
Ala 5	-	expression tag
Leu 6	-	expression tag
Ser 7	-	expression tag
Tyr 8	-	expression tag
Ile 9	-	expression tag
Phe 10	-	expression tag
Cys 11	-	expression tag
Leu 12	-	expression tag
Val 13	-	expression tag
Phe 14	-	expression tag
Ala 15	-	expression tag
Asp 16	-	expression tag
Tyr 17	-	expression tag
Lys 18	-	expression tag
Asp 19	-	expression tag
Asp 20	-	expression tag
Asp 21	-	expression tag
Asp 22	-	expression tag
Lys 23	-	expression tag
His 24	-	expression tag
Gly 25	-	expression tag
Ser 26	-	expression tag

R: 25 - 474 (UniProt: P30988)

PDB	External Database	Details
Met -7	-	expression tag
Lys -6	-	expression tag
Thr -5	-	expression tag
Ile -4	-	expression tag
Ile -3	-	expression tag
Ala -2	-	expression tag
Leu -1	-	expression tag
Ser 0	-	expression tag
Tyr 1	-	expression tag
Ile 2	-	expression tag
Phe 3	-	expression tag
Cys 4	-	expression tag
Leu 5	-	expression tag
Val 6	-	expression tag
Phe 7	-	expression tag
Ala 8	-	expression tag
Asp 9	-	expression tag
Tyr 10	-	expression tag
Lys 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Leu 16	-	expression tag
Glu 17	-	expression tag
Val 18	-	expression tag
Leu 19	-	expression tag
Phe 20	-	expression tag
Gln 21	-	expression tag
Gly 22	-	expression tag
Pro 23	-	expression tag
Ala 24	-	expression tag
Leu 447	Pro 447	conflict
Pro 475	-	expression tag
Ala 476	-	expression tag
Gly 477	-	expression tag
Leu 478	-	expression tag
Glu 479	-	expression tag
Val 480	-	expression tag
Leu 481	-	expression tag
Phe 482	-	expression tag
Gln 483	-	expression tag
Gly 484	-	expression tag
Pro 485	-	expression tag
His 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag
His 492	-	expression tag
His 493	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	186552.0
Non-Polymers*	Number of molecules	10
	Total formula weight	2919.2
All*	Total formula weight	189471.2

*Water molecules are not included.